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Metal-binding proteins and peptides in the aquatic fungi Fontanospora fusiramosa and Flagellospora curta exposed to severe metal stress

2006Journal article Restricted access
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dc.contributor.authorGuimarães-Soares, Luís
dc.contributor.authorFelícia, Helena
dc.contributor.authorBebianno, Maria João
dc.contributor.authorCássio, Fernanda
dc.date.accessioned2020-05-11T09:49:11Z
dc.date.available2020-05-11T09:49:11Z
dc.date.issued2006
dc.description.abstractThe production of thiol-containing proteins/peptides and its role in metal-binding was examined in the aquatic hyphomycetes Fontanospora fusiramosa and Flagellospora curta exposed to Cu, Cd, or Zn at concentrations inhibiting the biomass production in 80%. Heat-treated cell-free extracts were separated by size-exclusion chromatography and the thiol and metal content in the fractions was determined. F. curta, the species tolerant to metals, showed higher absolute levels of thiol compounds, which bound higher amounts of Cu and Cd than F. fusiramosa. Peptides with very low molecular weight (<9 kDa), most likely glutathione and phytochelatins, were the major Cu- and Zn-binding components in both species of aquatic hyphomycetes. In most cases, proteins with high molecular weight (>26 kDa) were induced by metal ions and they were the major Cd-binding component in both species. Proteins with characteristics of metallothioneins were also induced by exposure to metals in both species, but they showed a minor role in metal-binding, suggesting they might have other functions in fungal cells.pt_PT
dc.description.sponsorshipThis work was supported by Portuguese project grant POCTI/34024/BSE/2000.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.scitotenv.2006.09.017pt_PT
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/13868
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAscomycotapt_PT
dc.subjectCadmiumpt_PT
dc.subjectCarrier proteinspt_PT
dc.subjectCopperpt_PT
dc.subjectPeptidespt_PT
dc.subjectSulfhydryl compoundspt_PT
dc.subjectZincpt_PT
dc.titleMetal-binding proteins and peptides in the aquatic fungi Fontanospora fusiramosa and Flagellospora curta exposed to severe metal stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F3193%2F2000/PT
oaire.citation.endPage156pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage148pt_PT
oaire.citation.titleScience of the Total Environmentpt_PT
oaire.citation.volume372pt_PT
oaire.fundingStreamSFRH
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublication9345735b-34b9-4e88-9e1d-6d1225f8ac3d
relation.isProjectOfPublication.latestForDiscovery9345735b-34b9-4e88-9e1d-6d1225f8ac3d

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