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Novel triblock co-polymer nanofibre system as an alternative support for embryonic stem cells growth and pluripotency

2016Journal article Open Access
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dc.contributor.authorPerestrelo, Ana Rubina
dc.contributor.authorMouffouk, Fouzi
dc.contributor.authorCosta, Ana M. Rosa da
dc.contributor.authorBelo, José A.
dc.date.accessioned2016-01-06T14:21:43Z
dc.date.available2016-01-06T14:21:43Z
dc.date.issued2016
dc.description.abstractConventionally, embryonic stem cells (ESCs) are cultured on gelatin or over a mitotically inactivated monolayer of mouse embryonic fibroblasts (MEFsi). Considering the lack of versatile, non-animal-derived and inexpensive materials for that purpose, we aimed to find a biomaterial able to support ESC growth in a pluripotent state that avoids the need for laborious and time-consuming MEFsi culture in parallel with mouse ESC (mESC) culture. Undifferentiated mESCs were cultured in a new nanofibre material designed for ESC culture, which is based on the self-assembly of a triblock co-polymer, poly(ethyleneglycol-β-trimethylsilyl methacrylate-β-methacrylic acid), conjugated with the peptide glycine-arginine-glycine-aspartate-serine, to evaluate its potential application in ESC research. The morphology, proliferation, viability, pluripotency and differentiation potential of mESCs were assessed. Compared to conventional stem cell culture methodologies, the nanofibres promoted a higher increase in mESCs number, enhanced pluripotency and were able to support differentiation after long-term culture. This newly developed synthetic system allows the elimination of animal-derived matrices and provides an economic method of ESC culture, made of a complex network of nanofibres in a scale similar to native extracellular matrices, where the functional properties of the cells can be observed and manipulated.pt_PT
dc.identifier.citationJ Tissue Eng Regen Med (in press)pt_PT
dc.identifier.doihttp://dx.doi.org/ 10.1002/term.1838pt_PT
dc.identifier.issn1932-6254
dc.identifier.otherAUT: AMC01695; JBE01557;
dc.identifier.urihttp://hdl.handle.net/10400.1/7387
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationSTUDY OF THE APPLICATION POTENTIAL OF A NEW CLASS OF 3D SCAFFOLD POLYMERIC NANOFIBERS IN STEM CELL RESEARCH
dc.relationStrategic Project - LA 23 - 2011-2012
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/term.1838/pdfpt_PT
dc.subjectEmbryonic stem cellspt_PT
dc.subjectPolymeric nanofibrespt_PT
dc.subjectPluripotencypt_PT
dc.subjectEmbryonic stem cell culturept_PT
dc.subjectGrowth supportpt_PT
dc.subjectGelatin substitutept_PT
dc.titleNovel triblock co-polymer nanofibre system as an alternative support for embryonic stem cells growth and pluripotencypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSTUDY OF THE APPLICATION POTENTIAL OF A NEW CLASS OF 3D SCAFFOLD POLYMERIC NANOFIBERS IN STEM CELL RESEARCH
oaire.awardTitleStrategic Project - LA 23 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBD%2F65624%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0023%2F2011/PT
oaire.citation.titleJournal of Tissue Engineering and Regenerative Medicinept_PT
oaire.fundingStreamPIDDAC
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRosa da Costa
person.familyNameBelo
person.givenNameAna M
person.givenNameJosé A.
person.identifier305029
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridE-2165-2012
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id6602141392
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscovery0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isProjectOfPublication40d6065a-dc53-4385-81af-a2f85a1c2594
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