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Transcriptomics provides insight into Mytilus galloprovincialis (Mollusca: Bivalvia) mantle function and its role in biomineralisation

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dc.contributor.authorYarra, T.
dc.contributor.authorChurcher, Allison
dc.contributor.authorFelix, R. C.
dc.contributor.authorClark, M. S.
dc.contributor.authorPower, Deborah
dc.date.accessioned2017-04-07T15:56:34Z
dc.date.available2017-04-07T15:56:34Z
dc.date.issued2016
dc.description.abstractThe mantle is an organ common to all molluscs and is at the forefront of the biomineralisation process. The present study used the Mediterranean mussel (Mytilus galloprovincialis) as a model species to investigate the structural and functional role of the mantle in shell formation. The transcriptomes of three regions of the mantle edge (umbo to posterior edge) were sequenced using Illumina technology which yielded a total of 61,674,325 reads after adapter trimming and filtering. The raw reads assembled into 179,879 transcripts with an N50 value of 1086 bp.A total of 1363 transcripts (321, 223 and 816 in regions 1, 2 and 3, respectively) that differed in abundance in the three mantle regions were identified and putative function was assigned to 54% using BLAST sequence similarity searches (cut-off less than 1 e(-10)). Morphological differences detected by histology of the three mantle regions was linked to functional heterogeneity by selecting the top five most abundant Pfam domains in the annotated 1363 differentially abundant transcripts across the three mantle regions. Calcium binding domains dominated region two (middle segment of the mantle edge). Candidate biomineralisation genes were mined and tested by qPCR. This revealed that Flp-like, a penicillin binding protein potentially involved in shell matrix maintenance of the Pacific oyster (Crassostrea gigas), had significantly higher expression in the posterior end of the mantle edge (region one). Our findings are intriguing as they indicate that the mantle edge appears to be a heterogeneous tissue, displaying structural and functional bias. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier.doihttp://dx.doi.org/10.1016/j.margen.2016.03.004
dc.identifier.issn1874-7787
dc.identifier.urihttp://hdl.handle.net/10400.1/9454
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCHARACTERIZATION OF CHEMOSENSORY G PROTEIN-COUPLED RECEPTORS IN A BASAL METAZOAN MAY REVEAL THE ORIGIN OF CELL-CELL COMMUNICATION
dc.relation.isbasedonWOS:000379376100006
dc.subjectRNA-seq
dc.subjectGene expression
dc.subjectMulti-functional
dc.subjectPfam
dc.subjectTrinity
dc.titleTranscriptomics provides insight into Mytilus galloprovincialis (Mollusca: Bivalvia) mantle function and its role in biomineralisation
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCHARACTERIZATION OF CHEMOSENSORY G PROTEIN-COUPLED RECEPTORS IN A BASAL METAZOAN MAY REVEAL THE ORIGIN OF CELL-CELL COMMUNICATION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F85408%2F2012/PT
oaire.citation.endPage45
oaire.citation.startPage37
oaire.citation.titleMarine Genomics
oaire.citation.volume27
oaire.fundingStream5876
oaire.fundingStreamOE
person.familyNameChurcher
person.familyNamePower
person.givenNameAllison
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1902-3002
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-6169-2013
person.identifier.scopus-author-id15062343200
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc7392254-eaa2-4cd6-86b2-d350ab7c8d82
relation.isProjectOfPublication868b4818-3efa-4edb-9202-c464d64fd38f
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relation.isProjectOfPublication.latestForDiscovery868b4818-3efa-4edb-9202-c464d64fd38f

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