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Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization

2013-12Journal article Open Access
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dc.contributor.authorVijayakumar, Parameswaran
dc.contributor.authorLaizé, Vincent
dc.contributor.authorCardeira Da Silva, JoĂŁo
dc.contributor.authorTrindade, Marlene
dc.contributor.authorCancela, M. Leonor
dc.date.accessioned2018-12-07T14:52:53Z
dc.date.available2018-12-07T14:52:53Z
dc.date.issued2013-12
dc.description.abstractMechanisms of bone formation and skeletal development have been successfully investigated in zebrafish using a variety of in vivo approaches, but in vitro studies have been hindered due to a lack of homologous cell lines capable of producing an extracellular matrix (ECM) suitable for mineral deposition. Here we describe the development and characterization of a new cell line termed ZFB1, derived from zebrafish calcified tissues. ZFB1 cells have an epithelium-like phenotype, grow at 28 degrees C in a regular L-15 medium supplemented with 15% of fetal bovine serum, and are maintained and manipulated using standard methods (e.g., trypsinization, cryopreservation, and transfection). They can therefore be propagated and maintained easily in most cell culture facilities. ZFB1 cells show aneuploidy with 2n=78 chromosomes, indicative of cell transformation. Furthermore, because DNA can be efficiently delivered into their intracellular space by nucleofection, ZFB1 cells are suitable for gene targeting approaches and for assessing gene promoter activity. ZFB1 cells can also differentiate toward osteoblast or chondroblast lineages, as demonstrated by expression of osteoblast- and chondrocyte-specific markers, they exhibit an alkaline phosphatase activity, a marker of bone formation in vivo, and they can mineralize their ECM. Therefore, they represent a valuable zebrafish-derived in vitro system for investigating bone cell differentiation and extracellular matrix mineralization.
dc.description.sponsorshipPEst-C/MAR/LA0015/2011]; project [FP7/227799]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1089/zeb.2012.0833
dc.identifier.issn1545-8547
dc.identifier.issn1557-8542
dc.identifier.urihttp://hdl.handle.net/10400.1/11250
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMary Ann Liebert
dc.relationDevelopment and characterization of zebrafish stem cells: implications for transgenesis - FishCell
dc.relationStrategic Project - LA 15 - 2011-2012
dc.relationMOLECULAR DETERMINANTS OF TISSUE MINERALIZATION IN FISH: A FUNCTIONAL GENOMIC APPROACH
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlkaline phosphatase activity
dc.subjectDanio rerio
dc.subjectStem cells
dc.subjectGene expression
dc.subjectOsteoblastic differentiation
dc.subjectRetinoic acid
dc.subjectSparus aurata
dc.subjectCollagen gene
dc.subjectGla protein
dc.subjectFish
dc.titleDevelopment of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment and characterization of zebrafish stem cells: implications for transgenesis - FishCell
oaire.awardTitleStrategic Project - LA 15 - 2011-2012
oaire.awardTitleMOLECULAR DETERMINANTS OF TISSUE MINERALIZATION IN FISH: A FUNCTIONAL GENOMIC APPROACH
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F105313%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Projectos de I&D em todos os DomĂ­nios CientĂ­ficos - 2008/PTDC%2FMAR%2F105313%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FMAR%2FLA0015%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F39189%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBPD%2F39189%2F2007/PT
oaire.citation.endPage509
oaire.citation.issue4
oaire.citation.startPage500
oaire.citation.titleZebrafish
oaire.citation.volume10
oaire.fundingStream3599-PPCDT
oaire.fundingStreamConcurso para Projectos de I&D em todos os DomĂ­nios CientĂ­ficos - 2008
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamSFRH
oaire.fundingStreamPIDDAC
person.familyNameVijayakumar
person.familyNameLaizé
person.familyNameCardeira da Silva
person.familyNameTrindade
person.familyNameCancela
person.givenNameParameswaran
person.givenNameVincent
person.givenNameJoĂŁo
person.givenNameMarlene
person.givenNameM. Leonor
person.identifier.ciencia-idDC15-86F7-3AAC
person.identifier.orcid0000-0002-1345-586X
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3722-8810
person.identifier.orcid0000-0001-7072-3392
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridE-5298-2017
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id8973850300
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id55224020100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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