Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/2027
Título: Structure, tissue distribution and estrogen regulation of splice variants of the sea bream estrogen receptor alpha gene
Autor: Pinto, Patricia
Teodósio, H. R.
Socorro, S.
Power, Deborah
Canario, Adelino V. M.
Data: Jul-2012
Editora: Elsevier
Resumo: Estrogen actions are mainly mediated by specific nuclear estrogen receptors (ERs), for which different genes and a diversity of transcript variants have been identified, mainly in mammals. In this study, we investigated the presence of ER splice variants in the teleost fish gilthead sea bream (Sparus auratus), by comparison with the genomic organization of the related species Takifugu rubripes. Two exon2-deleted ERα transcript variants were isolated from liver cDNA of estradiol-treated fish. The ΔE2 variant lacks ERα exon 2, generating a premature termination codon and a putative C-terminal truncated receptor, while the ΔE2,3* variant contains an in-frame deletion of exon 2 and part of exon 3 and codes for a putative ERα protein variant lacking most of the DNA-binding domain. Both variants were expressed at very low levels in several female and male sea bream tissues, and their expression was highly inducible in liver by estradiol-17β treatment with a strong positive correlation with the typical wild-type (wt) ERα response in this tissue. These findings identify novel estrogen responsive splice variants of fish ERα, and provide the basis for future studies to investigate possible modulation of wt-ER actions by splice variants.
Peer review: yes
URI: http://hdl.handle.net/10400.1/2027
Aparece nas colecções:CCM2-Artigos (em revistas ou actas indexadas)

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