Browsing by Author "Acosta, N. G."
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- In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexesPublication . Reis, Diana; Acosta, N. G.; Almansa, E.; Navarro, J. C.; Tocher, D. R.; Monroig, O.; Andrade, José Pedro; Sykes, António V.; Rodríguez, C.The highmortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36‰). Incubations were performed with gentle stirring at 21 °C for 6 h with 0.2 μCi (0.3 μM) of [1−14C]-labelled FA including 18:1n−9, 18:2n−6, 18:3n−3, 20:4n−6 (ARA), 20:5n−3 (EPA) or 22:6n−3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum albumin (BSA). A control treatmentwithout [1−14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1−14C]DHA and [1−14C]C18 FA substratesweremainly esterified into phosphatidylcholine, while [1−14C]ARA and [1−14C]EPA were esterified into phosphatidylethanolamine.
- In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlingsPublication . Reis, Diana; Rodriguez, C.; Acosta, N. G.; Almansa, E.; Tocher, D. R.; Andrade, José Pedro; Sykes, António V.The transition of Sepia officinalis culture to industrial large scale has been hampered due to bottlenecks related to the limited knowledge on nutritional physiology of the species. Determination of the endogenous ability of S. officinalis hatchlings to metabolise unsaturated fatty acids (FA) may provide new insight on the capability of hatchlings to biosynthesise different FA, as well as lipid classes containing essential fatty acids (EFA). In the present study, cuttlefish hatchlings were incubated with [1-C-14] FA including C18 FA (18:1n-9, 18:2n-6, 18:3n-3) and long-chain polyunsaturated fatty acids (LC-PUFA) (20: 4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA)), which were added individually as potassium salts bound to bovine serumalbumin. As a result, it was possible to investigate the in vivo FAmetabolismof S. officinalis hatchlings by following the incorporation of specific [1-C-14] FA, which points to the suitability of this methodology to study lipid metabolism of newly hatched cephalopods. The majority of radioactivity incorporated was recovered esterified into polar lipids (PL). A pattern was detected, where [1-C-14] DHA, [1-C-14] C18 FA and their metabolic products were preferentially esterified into phosphatidylcholine, whereas [1-C-14] ARA and [1-C-14] EPA were mainly esterified into phosphatidylethanolamine. [1-C-14] C18 FA were the most transformed FA with several metabolites produced by elongation and possible desaturation being obtained. As a contrary the radioactivity incorporated into hatchling total lipid (TL) from supplemented [1-C-14] LC-PUFA only one elongation product was recovered from the three substrates, except for [1-C-14] ARA, where an unidentified product was also detected. The present in vivo results indicated that S. officinalis hatchlings may have capability for the first steps in the biosynthesis of ARA and EPA from 18: 2n-6 and 18: 3n-3, respectively, including the existence of a desaturase potentially involved. Nonetheless, considering the low desaturation rates detected, this process may not be sufficient to cover EFA demands during development of this species. Therefore, dietary ARA and EPA, as well as DHA, should be supplied during the hatchling stage of Sepia. While designing an inert diet, which ensures normal growth and development of this species during the hatchling stage, the C18 FA and LC-PUFA levels and ratios should be considered, since the esterification pattern detected in the present study suggested competition between these FA for esterification into specific lipid classes. Moreover, considering the observed esterification pattern of LC-PUFA into different lipid classes, it is likely that the DHA/EPA/ARA ratio, rather than DHA/EPA or EPA/ARA ratios, would be of great importance for S. officinalis hatchling development. (C) 2015 Elsevier B.V. All rights reserved.