Percorrer por autor "Apostolova, E."
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- Genetic diversity assessment of the almond (Prunus dulcis (Mill.) DA Webb) traditional germplasm of Algarve, Portugal, using molecular markersPublication . Cabrita, L.; Apostolova, E.; Neves, Alcinda; Marreiros, A.; Leitão, JoséIn this study, 123 almond (Prunus dulcis (Mill.) D. A. Webb) trees identified among traditional orchards in the Algarve region and 53 trees of the local field collection managed by the regional office of the Portuguese Ministry of Agriculture (DRAALG) were assessed using isozyme, inter- single sequence repeat and simple sequence repeat or microsatellite techniques for the evaluation of genetic diversity and genetic relatedness and identification of new accessions for the field collection. The isozyme analysis allowed the distribution of the 176 plants into 13 different classes of enzyme similarity, while the use of DNA markers increased the distribution of the analysed trees among 140 discriminating DNA patterns. Multiple cases of homonymy and synonymy were identified in the local germplasm. Some traditional varieties, such as Lourencinha, appeared to be relatively homogeneous, while other local denominations, e.g. Galamba, included diverse genotypes. Of the 13 commercial varieties analysed in this study, 11 assembled in one major cluster clearly differentiated from the majority of the local genotypes. These results reinforced the perception that the Algarve traditional germplasm constitutes an important repository of genetic diversity, eventually carrying alleles of high agricultural interest such as the recently identified Phomopsis resistance in the traditional variety Barrinho Grado.
- Identification of RAPD markers linked to the Fin and P loci in Phaseolus vulgaris L.Publication . Apostolova, E.; Svetleva, D.; Carlier, Jorge; Leitão, JoséBulked segregant analysis was used to identify random amplified polymorphic DNA RAPD markers linked to the genes for indeterminate growth habit (Fin) and seed color (P) in common bean (Phaseolus vulgaris L.) using an F2 population derived from the cross A 195 × Kristal 137. The variety A 195 is characterized by determinate growth habit and brown colored seeds, while Kristal 137 exhibits indeterminate growth habit and white colored seeds. Among the 140 RAPD primers used to discriminate between two parental genotypes and between two bulks of DNA, respectively, from determinate and indeterminate plants, only 64 (45.71%) amplified polymorphic markers. Nineteen primers amplified clear polymorphic bands and were further tested among 100 F 2 plants. The analysis of the genetic linkage between analyzed molecular markers and the loci for indeterminate growth habit and seed color was performed using the program Join Map 3.0. The logarithm of odds (LOD) of 8.0 and 10 were used as thresholds for linkage grouping, respectively of locus Fin and locus P. One RAPD marker, AA18-600-P1, was found linked to the Fin locus that determines plant habit type and mapped at 17.6 cM apart from it (rec. freq. = 0.162). Five RAPD markers were found linked to the P locus, with distances to this locus varying from 4.1 cM (marker AB18 1500-P2, rec. freq.= 0.034) to 42.6 cM (marker Q03700-P1).