Percorrer por autor "Attanayake, Attanayake Mudiyanselage Ransirini Chandrika"
A mostrar 1 - 1 de 1
Resultados por página
Opções de ordenação
- Distribution of antibiotic resistance genes in soil-plant ecosystem under manure fertilizationPublication . Attanayake, Attanayake Mudiyanselage Ransirini Chandrika; Chícharo, Luis; Urbaniak, Magdalena; Mierzejewska, ElżbietaThe global demand for food production has enhanced the application of animal manure as an organic fertilizer for sustainable soil management practices. However, recent studies have highlighted the problem of the occurrence of veterinary antibiotics in animal manure and the risk of the development of antibiotic resistance in terrestrial and aquatic ecosystems. This study investigates the spread of antibiotic resistance genes (ARGs) in the microbiome of manure, soil, and plant-associated bacteria of cherry radish following the application of three kinds of animal manure (turkey, cow, and pig). A 45-day pot experiment was conducted, involving the sampling of manure-amended soil, rhizospheric bacteria, and endophytic bacteria inhabiting cherry radish roots and leaves. The samples were used to analyze the presence of 16S rRNA genes and 21 ARGs belonging to 7 Antibiotic Resistance Phenotypes (ARPs). Soil samples from the end of the experiment (45 day) were additionally used to check the influence of manure on the functional diversity of the soil microbiome (Biolog Ecoplates) and determine soil dehydrogenase activity.