Browsing by Author "Authier, Tatiana Marine da Silva"
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- Molecular and functional analysis of mutations in the transcription factor ZNF687 associated to Paget disease of bonePublication . Authier, Tatiana Marine da Silva; Cancela, Leonor; Conceição, NatérciaThe zinc finger protein ZNF687 is a transcription factor containing various Cys2-His2 zinc finger domains that has been related to Paget’s disease of bone (PDB) associated with giant cell tumor of bone. Recently, four mutations have been independently identified in a Southern Italian population, and were determined as predisposal (p.Ser242Ile, p.Pro665Leu, and p.Gln784Glu,) or causal (p.Pro937Arg) for development of PDB. Moreover, it has been suggested that ZNF687 plays an important role in bone metabolism in both human and zebrafish, and that the function of this protein might be conserved throughout evolution. Nonetheless, the mechanism by which ZNF687 affects bone metabolism, and hence, PDB, remains to be elucidated. To contribute to respond to these questions we have proposed to perform in silico analysis in order to investigate how these four mutations could affect the protein conformation and function, and in vitro analyses to evaluate how mutant ZNF687 (p.Pro937Arg, mediated by site-directed mutagenesis), ZNF687 overexpression (mediated by transient transfection) and ZNF687 knock-down (mediated by CRISPR-Cas9), could affect the expression of genes involved in bone metabolism. We also assessed the mineralization process of knock-down clones and evaluated specific gene expression. Lastly, comparative in silico and in vitro analyses were performed in order to define the usefulness of zebrafish as a biological model for ZNF687 study. Therefore, the mutation analysis suggested alterations in protein-protein and protein-acid nucleic interactions that might distort ZNF687 target genes expression and lead to PDB. Moreover, preliminary results suggested that ZNF687 might regulate genes involved in osteoblastogenesis and osteoclastogenesis, such as RUNX2, OSX, RANK, SQSTM1 and OPTN, while its role in mineralization remained uncleared. Finally, by identifying significant similarities in genomic structure, protein domain, and molecular players affecting znf687a, znf687b and ZNF687 transcription, together with the identification and characterization of promoter’s regions that regulate the transcription of znf687a and human ZNF687 genes, we have confirmed that zebrafish is a useful biological model system to study ZNF687.