Percorrer por autor "Costa, Carlos Alexandre Rocha da"
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- Building hovenia dulcis pseudofruit quality from proteomic and metabolomic perspectivesPublication . Machado, Gilson Gustavo Lucinda; Eller, Elda; Ribeiro, Carlos Henrique Milagres; Costa, Carlos Alexandre Rocha da; Nascimento, Sidney Vasconcelos do; Nahon, Sayure Mariana Raad; Cavalcante, Alice de Paula de Sousa; Costa, Isa Rebecca Chagas da; Valadares, Rafael Borges da Silva; Carvalho, Elisângela Elena Nunes; Boas, Eduardo Valério de Barros VilasHovenia dulcis Thunb. (Japanese grape tree) pseudofruit exhibits a period of growth of 180 days, with maturation initiated between 120 and 150 days after anthesis (DAA). Effective ripening occurs 150 DAA, characterized by intense softening, pectic solubilization and starch-sugar conversion. Multi-omic analysis identified 496 proteins, with 222 being differentially expressed. Significant highlights included energy metabolism (Malate Dehydrogenases, Glyceraldehyde-3-phosphate Dehydrogenase, Pyruvate Kinase) and photosynthetic pathways (Ribulose-1,5-bisphosphate Carboxylase/Oxygenase, Photosystems I and II), indicating that ripening demands a high energy supply. Multivariate analysis stratified the development into three phases: chemical defense (S1-S2), metabolic transition (S3-S6), and sensory ripening (S7). The Variable Importance in Projection (VIP) score and Principal Component 1 (PC1) loadings confirmed that the transition is governed by changes in volatile compounds (2-heptanol), respiratory physiology, density, and Hue angle, with a central role for malate dehydrogenase. Pearson correlations revealed a coordinated system in which central metabolism (Glyceraldehyde-3- phosphate Dehydrogenase, Triose Phosphate Isomerase) is coupled with ethylene signaling (S-adenosylmethionine Synthetase) and antioxidant defenses (Superoxide Dismutase, Catalase, and Peroxiredoxin 2). It is concluded that H. dulcis development is sustained by a strategic proteometabolic network that redirects the investment from protective biomolecules toward the specialization of sensory attributes, defining the final quality of the pseudofruit.
- Metabolomics and proteomics of campomanesia pubescens fruits throughout developmental stagesPublication . Machado, Gilson Gustavo Lucinda; Costa, Carlos Alexandre Rocha da; Eller, Elda; Nascimento, Sidney Vasconcelos do; Nahon, Sayure Mariana Raad; Cavalcante, Alice de Paula de Sousa; Costa, Isa Rebecca Chagas da; Valadares, Rafael Borges da Silva; Carvalho, Elisângela Elena Nunes; Livramento, Kalynka Gabriella do; Vilas Boas, Eduardo Valério de BarrosThis study investigated the metabolic changes occurring during the ripening of Campomanesia pubescens fruits using metabolomics and proteomics approaches. Two alkaloids and nine phenolic compounds were identified, with emphasis on trigonelline and gallic acid. Proteomic analysis revealed 940 proteins, of which 452 showed differential expression, reflecting physiological changes, such as the reduced expression of ribulose-1,5- bisphosphate carboxylase/oxygenase, indicating low photosynthetic activity and the transition from chloroplasts to chromoplasts. The intensification of glycolysis was suggested by the increased expression of phosphoglycerate kinase and fructose-bisphosphate aldolase. The activation of enzymes of the phenolic compound pathway, such as phenylalanine ammonia-lyase and O-methyltransferase, indicated the accumulation of secondary metabolites essential for fruit defense and nutritional quality. The expression of enzymes in the ethylene pathway, such as SAM synthase, suggested their role in gabiroba ripening. The results highlight the interaction of metabolic pathways during fruit ripening.