Percorrer por autor "Costa, R."
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- Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal watersPublication . Hardoim, C. C. P.; Costa, R.; Araujo, F. V.; Hajdu, E.; Peixoto, R.; Lins, U.; Rosado, A. S.; van Elsas, J. D.Microorganisms can account for up to 60% of the fresh weight of marine sponges. Marine sponges have been hypothesized to serve as accumulation spots of particular microbial communities, but it is unknown to what extent these communities are directed by the organism or the site or occur randomly. To address this question, we assessed the composition of specific bacterial communities associated with Aplysina fulva, one of the prevalent sponge species inhabiting Brazilian waters. Specimens of A. fulva and surrounding seawater were collected in triplicate in shallow water at two sites, Caboclo Island and Tartaruga beach, Buzios, Brazil. Total community DNA was extracted from the samples using "direct" and "indirect" approaches. 16S rRNA-based PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the total bacterial community and of specific bacterial groups-Pseudomonas and Actinobacteria-revealed that the structure of these assemblages in A. fulva differed drastically from that observed in seawater. The DNA extraction methodology and sampling site were determinative for the composition of actinobacterial communities in A. fulva. However, no such effects could be gleaned from total bacterial and Pseudomonas PCR-DGGE profiles. Bacterial 16S rRNA gene clone libraries constructed from directly and indirectly extracted DNA did not differ significantly with respect to diversity and composition. Altogether, the libraries encompassed 15 bacterial phyla and the candidate division TM7. Clone sequences affiliated with the Cyanobacteria, Chloroflexi, Gamma-and Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were, in this order, most abundant. The bacterial communities associated with the A. fulva specimens were distinct and differed from those described in studies of sponge-associated microbiota performed with other sponge species.
- Metagenomic insights into the taxonomy, function, and dysbiosis of prokaryotic communities in octocoralsPublication . Keller-Costa, T.; Lago-Lestón, A.; Saraiva, J. P; Toscan, R.; Silva, S. G; Gonçalves, Jorge Manuel Santos; Cox, Cymon J.; Kyrpides, N.; Nunes da Rocha, U.; Costa, R.In octocorals (Cnidaria Octocorallia), the functional relationship between host health and its symbiotic consortium has yet to be determined. Here, we employed comparative metagenomics to uncover the distinct functional and phylogenetic features of the microbiomes of healthy Eunicella gazella, Eunicella verrucosa, and Leptogorgia sarmentosa tissues, in contrast with the microbiomes found in seawater and sediments. We further explored how the octocoral microbiome shifts to a pathobiome state in E. gazella.
- Prevalence and clinical characteristics of late Onset Axial Spondyloarthritis: Results from a multicentre nationwide studyPublication . Rocha, Margarida Lucas; Torres, R.; Ramiro, S.; Castro, A. M.; Neves, A.; Martins, A.; Chícharo, A. T.; Mendes, B.; Matos, C. O.; Soares, C.; Oliveira, C. P.; Parente, H.; Gomes, J. A. M.; Luís, M.; Santos, M.; Couto, M.; Bernardes, M.; Valente, P.; Costa, R.; Sousa, S.; Branco, J.; Pimentel-Santos, F.; Sepriano, A.Axial spondyloarthritis (axSpA) typically starts before the fourth decade oflife. Consistent with that, the Assessment of SpondyloArthritis international Society (ASAS)classification criteria for axSpA should be applied only in patients with chronic back pain startingbefore 45 years of age. It has, however, been suggested that axSpA can sometimes start later in lifewith a distinctive phenotype, the so-called ‘late onset axSpA’ (lo-axSpA). There is, nevertheless, onlylimited data in support of the existence of such phenotype. We aimed to evaluate the occurrence oflo-axSpA and if these patients differ from those with early onset axSpA (eo-axSpA).