Browsing by Author "Cusumano, Gaia"
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- Characterising the metabolomic diversity and biological potentials of extracts from different parts of two cistus species using UHPLC-MS/MS and In vitro techniquesPublication . Ahmed, Shakeel; Zengin, Gokhan; Selvi, Selami; Ak, Gunes; Cziáky, Zoltán; Jekő, József; Rodrigues, Maria João; Custódio, Luísa; Venanzoni, Roberto; Flores, Giancarlo Angeles; Cusumano, Gaia; Angelini, PaolaThis study investigates the biochemical composition and biological properties of different parts (leaves, roots, and twigs) of two Cistus species (Cistus monspeliasis and Cistus parviflorus). The extracts were analysed using UHPLC-MS/MS to determine their chemical profiling. A range of antioxidant assays were performed to evaluate the extract’s antioxidant capabilities. The enzyme inhibition studies focused on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, and α-glucosidase and tyrosinase. In addition, the study examined the antimicrobial effects on different bacteria and yeasts and evaluated the toxicity using the MTT assay. Quinic acid, citric acid, gallic acid, catechin, quercetin derivatives, kaempferol, myricetin, ellagic acid, prodelphinidins, procyanidins, scopoletin, and flavogallonic acid dilactone are the main bioactive compounds found in both species. In enzyme inhibition assays, C. monspeliasis roots exhibited significant activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with the values of 2.58 ± 0.02 mg GALAE/g and 11.37 ± 1.93 mg GALAE/g, respectively. Cytotoxicity studies showed mostly weak toxicity, with some samples moderately reducing viability in RAW and HepG2 cells. These findings underscore the diverse biochemical profiles and bioactive potential of Cistus species, suggesting their utility as natural sources of antioxidants and enzyme inhibitors for pharmaceutical and nutraceutical development.
- Small steps to the big picture for health‐promoting applications through the use of chickweed (Stellariamedia): In vitro, in silico, and pharmacologicalnetwork approachesPublication . Cusumano, Gaia; Angeles Flores, Giancarlo; Cetiz, Mehmet Veysi; Kurt, Umran; Ak, Gunes; Saka, Enver; Aly, Shaza H.; Eldahshan, Omayma A.; Singab, Abdel Nasser; Zengin, Gokhan; Senkardes, Ismail; Rodrigues, Maria J.; Custódio, Luísa; Emiliani, Carla; Angelini, PaolaStellaria media L., also called chickweed, is widespread in all parts of the world. In the present study, we investigated the bio-logical properties and chemical profiles of different extracts (ethyl acetate, ethanol, ethanol/water, and water) of S. media. Thechemical profiles were examined using UHPLC/MS/MS technique. Regarding the biological properties, antioxidant propertiesas well as enzyme-inhibiting and cytotoxic effects of the extracts were demonstrated by in vitro methods. To obtain further in-formation about the structure-ability relationship, network pharmacology and molecular docking were also performed. Twelvephenolic compounds were identified in the extracts and most of them were flavonoids (apigenin, kaempferol derivatives, etc.).The water extract showed the best free radical scavenging activity, while the ethanol was the most active in reducing power tests.When inhibiting AChE, the ethyl acetate extract showed the best inhibitory effect. The water extract has a good cytotoxic effecton HepG2 (cell viability: 33.9% at a concentration of 100 g/mL). The analysis, performed using the STRING database, includedthese 45 cancer-associated targets. The identified hub genes were TP53, CDKN2A, PTEN, KRAS, and HRAS. In moleculardocking analysis, acacetin- O-hexoside- O-deoxyhexoside and napigenin-7- O-hexoside exhibit remarkable binding energies withproteins. Consequently, S. media can be potential raw materials for designing functional formulations in the pharmaceutical,nutraceutical, and cosmeceutical industries.