Browsing by Author "Elisiário, P. J."
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- Identification of mandarin hybrids by isozyme and RAPD analysisPublication . Elisiário, P. J.; Justo, E. M.; Leitão, J. M.A cross between the monoembryonic Clementine MA3 SRA 649 used as seed parent and the polyembryonic Carvalhais mandarin used as pollen progenitor was carried out in the SRA INRA-CIRAD, S. Giuliano, Corsica. Cuttings of 61 plants putatively resulting from this cross were sent to the Centro de Citricultura, Fare, Portugal for further agronomic evaluation under field trials. Isozyme analysis allowed the identification of 59 plants as having sexual origin, however, two plants that showed isozyme patterns identical to their seed parent were impossible to identify as being either of zygotic or of somatic origin. Isozymes confirmed Carvalhais mandarin as the pollen parent of 42 progeny plants, while RAPD analysis confirmed Carvalhais paternity over the remaining 19 plants. A fast and low pollutant method for extraction of Citrus total genomic DNA suitable for RAPD analysis is reported. (C) 1999 Elsevier Science B.V. All rights reserved.
- Isozyme analysis revealed that the Portuguese mandarin 'Carvalhais' originated as a single clonePublication . Elisiário, P. J.; Santos, G. G.; Guerreiro, A. R.; Ollitrault, P.; Luro, F.; Leitão, J. M.'Carvalhais' mandarin is a well-known Portuguese mandarin used in several Citrus breeding programmes throughout the world. The first step in the implementation of a breeding programme of this variety was to screen for its natural genetic variability. A significant number of adult trees and young seedlings were submitted to isozyme analysis. For better interpretation of the isozyme patterns of 'Carvalhais', several plants resulting from crosses between this Portuguese mandarin cultivar and the monoembryonic mandarin cultivar Clementine MA3 SRA 649, were also analysed. All the analysed trees of 'Carvalhais' showed similar monomorphic patterns for each isozyme system, indicating that this mandarin cultivar originated as a single initial clone. Young seedlings exhibited monomorphic isozyme patterns identical to the adult plants revealing a high level of apomixis in this cultivar. (C) 1999 Elsevier Science B.V. All rights reserved.