Browsing by Author "Elrawy, Abdelrahman Khaled Fouad"
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- Method development and application for screening and quantification of plasticizers in mussels by lc-hrmsPublication . Elrawy, Abdelrahman Khaled Fouad; Silva, José Paulo DaPlasticizers are widely used in the polymer industry to enhance flexibility and durability of plastics materials. However, their widespread use and effects raise significant environmental and public health concerns. Sensitive and accurate analytical methods are necessary to screen and determine their concentration in the environment and food products. A liquid chromatography coupled to high resolution mass spectrometry method (LC-HRMS), with Atmospheric Pressure Chemical Ionization (APCI), was implemented and optimized for quantification of Dipentyl phthalate (DPP), benzyl butyl phthalate (BBP), bis(2-ethylhexyl) phthalate (DEHP), Dicyclohexyl phthalate (DCHP), Diethyl Phthalates (DEP), Diisobutyl phthalate (DIBP), and Di-n-octyl Phthalate (DNOP) and screening of other phthalates in mussels. Different extraction solvents and acquisition conditions were tested. Extraction with dichloromethane followed by evaporation and resuspension in methanol was chosen for sample preparation procedure. All Ion Fragmentation (AIF) was selected for screening while quantification was performed using accurate mass-extracted ion chromatograms (AM-XIC) taken from Full Scan profiles. The limits of quantification (LOQs) of the target analytes after optimization was ranged from 2.59 to 12.99 μg/kg Average recoveries of the target analytes typically ranged from 46.39% to 110.86% for 500 nM and 70.02% to 90.76% for 50 nM. The matrix effects ranged from 29.50 to 192.94%. The method was successfully applied for screening of phthalates plasticizers in mussels and sediments samples collected from various locations in Macau and Algarve and was useful for the fast screening and quantifying of various phthalates. Our results suggest the use of AIF acquisition for screening and AM-XIC for quantification. Analysis using LC-HRMS provided reliable performance as well as high sensitivity and selectivity in complex samples as biological tissues and sediments.