Browsing by Author "Godfroid, Edmond"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
- Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomiPublication . Coelho, A. C.; Cravador, A.; Bollen, A.; Ferraz, J. F. P.; Moreira, A. C.; Fauconnier, A.; Godfroid, EdmondIn response to the need for a faster, more reliable method for identifying Phytophthora cinnamomi in cork oak soils in Portugal, a simple, fast, sensitive molecular identification method is described. It is based on a colorimetric assay which involves an oligonucleotide capture probe covalently immobilised on microtitration wells, a multi-biotinylated oligonucleotide detection probe and the PCR-amplified target DNA. The target DNA is a 349 bp DNA fragment partially covering the 3'-translated and 3'- untranslated regions of the cinnamomin gene. When the specificity of the PCR reaction was evaluated in vitro using isolates of P. cinnamomi and eight other Phytophthora species, including the related P. cambivora, it was specific to P. cinnamomi. When 30 isolates of P. cinnamomi from oak roots in southern Portugal were assayed, 26 gave a strong positive response. The assay has a sensitivity of about 2±5 genome equivalents of P. cinnamomi. The reason for the negative response of four isolates remains unclear.
- Identification of an elicitin gene cluster in Phytophthora cinnamomiPublication . Duclos, J.; Fauconnier, A.; Coelho, A. C.; Bollen, A.; Cravador, A.; Godfroid, EdmondElicitins are a group of highly conserved proteins secreted by species of Phytophthora and a species of the related genus Pythium, Pythium vexans. Some of these proteins act as inducers of the necrotic hypersensitive-like response and the associated systemic acquired resistance phenomenon, in some species. We cloned and characterised the cinnamomin-beta and -alpha genes and two related elicitin genes from Phytophthora cinnamomi. These four open reading frames (ORFs) are clustered in tandem pairs. Two out of these four genes present homologies with the basic and acidic elicitin groups; but the two others encode, if expressed, elicitin isoforms exhibiting homologies with the class II of highly acidic elicitins.
- Identification of an elicitin gene cluster in Phytophthora cinnamomi and analysis of the necrotic activity of a purified recombinant beta-cinnamominPublication . Duclos, J.; Aurélio, M.; Graca, J.; Coelho, A. C.; Fauconnier, A.; Jacquet, Alain; Bollen, A.; Cravador, A.; Biemans, R.; Godfroid, EdmondThe oomycetous fungus Phytophthora cinnamoni plays and important role in the necrotic activity observed on feeder root of cork oaks (Quercus suber L.) and eucalyptus (eucalyptus marginata Donn. Ex. Sm.)trees reducing the capacity of these hosts to absorb water and nutrients.