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Percorrer por autor "Igbodo, Benjamin"

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  • Implementation of standard methods for parameters routinely monitored in wastewater treatment plants and application in bioaugmentation with mycolicibacterium sp. In granular sludge reactors
    Publication . Igbodo, Benjamin; Costa, Maria Clara; Carliar, Jorge
    The effective treatment of domestic wastewater is pivotal for the health and sanitation status of water resources and wastewater reclamation campaigns. Thus, this study was aimed at evaluating the performance of Aerobic granular Sludge (AGS) in the treatment of synthetic wastewater during a bacteria bioaugmentation (Mycolicibacterium spp strain) experiment using laboratory-scale Sequencing Batch Reactors (SBRs). The routine parameters evaluated using UV–visible spectrophotometry methods were Total Nitrogen (TN), ammonia nitrogen (NH3 - N), nitrate nitrogen (NO3 - –N), phosphate phosphorus (PO4 3- - P) and Chemical Oxygen Demand (COD), on samples from the influents and effluents of the SBRs. The routine parameters evaluated using UV–visible spectrophotometry methods were Total Nitrogen (TN), ammonia nitrogen (NH3 - N), nitrate nitrogen (NO3- – N), phosphate phosphorus (PO43- - P) and Chemical Oxygen Demand (COD), on samples from the influents and effluents of the SBRs. Nitrite nitrogen (NO2- - N) was evaluated by mass balance and pH was measured using a benchtop pH meter. The individual standard calibration curves for the parameters had regression coefficients > 0.995 and the LOD (for qualitative analysis) and LOQ (for quantitative analysis) values were compatible with the concentrations of the samples analyzed while the validation process (using reference materials) yielded relative accuracy > 94 %.
    2023-09-15Dissertação de mestrado Acesso aberto Ver mais
  • Studies on the bioaugmentation of mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation
    Publication . Carlier, Jorge; Lara-Moreno, Alba; Igbodo, Benjamin; Costa, Maria Clara
    This study aimed to evaluate for the first time the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in Sequencing Batch Reactors (SBRs) with Aerobic Granular Sludge (AGS), and its effect on the biodegradation of Paracetamol, also known as Acetaminophen and N -acetyl-paraaminophenol (APAP). The bioaugmentation was effective and persisted for at least nine days after five inoculations performed in 24 days (relative abundance of M. aubagnense was 0.13 ± 0.05 % in the test reactors and 0.0079 ± 0.0008 % in the control reactors) and for eight days after seven inoculations performed in 40 days (relative abundance of M. aubagnense was 0.04 ± 0.02 % in the tests and 0.0005 ± 0.0005 % in the controls). In what concerns APAP biodegradation, the results showed a faster removal of its transformation products Hydroquinone (HQ), 2,5-dihydroxy-1,4- benzoquinone (2,5-HO-BQ) and 1,4-benzoquinone (BQ) in the bioreactors bioaugmented with the bacterial strain M. aubagnense HPB1.1 (59 % or 85 % of HQ, 67 % or 85 % of 2,5-HO-BQ and 75 % or 82 % of BQ removals, respectively for assay 1 or assay 2) in comparison to the nonbioaugmented bioreactors (15 % or 31 % of HQ, 36 % or 63 % of 2,5-HO-BQ and no removal of BQ, also for assy 1 and 2, respectively). Regarding the effect on organics and nutrients treatment, overall, the SBR conditions favored ammonia, nitrites, and organics removal. Yet, the conditions did not allow complete denitrification nor higher assimilation than release of PO4 3-.
    2025-05Artigo científico Acesso aberto Ver mais