Browsing by Author "Jacquet, Alain"
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- EAACI molecular allergology user's guide 2.0Publication . Dramburg, Stephanie; Hilger, Christiane; Santos, Alexandra F.; de las Vecillas, Leticia; Aalberse, Rob C.; Acevedo, Nathalie; Aglas, Lorenz; Altmann, Friedrich; Arruda, Karla L.; Asero, Riccardo; Ballmer‐Weber, Barbara; Barber, Domingo; Beyer, Kirsten; Biedermann, Tilo; Bilo, Maria Beatrice; Blank, Simon; Bosshard, Philipp P.; Breiteneder, Heimo; Brough, Helen A.; Bublin, Merima; Campbell, Dianne; Caraballo, Luis; Caubet, Jean Christoph; Celi, Giorgio; Chapman, Martin D.; Chruszcz, Maksymilian; Custovic, Adnan; Czolk, Rebecca; Davies, Janet; Douladiris, Nikolaos; Eberlein, Bernadette; Ebisawa, Motohiro; Ehlers, Anna; Eigenmann, Philippe; Gadermaier, Gabriele; Giovannini, Mattia; Gomez, Francisca; Grohman, Rebecca; Guillet, Carole; Hafner, Christine; Hamilton, Robert G.; Hauser, Michael; Hawranek, Thomas; Hoffmann, Hans Jürgen; Holzhauser, Thomas; Iizuka, Tomona; Jacquet, Alain; Jakob, Thilo; Janssen‐Weets, Bente; Jappe, Uta; Jutel, Marek; Kalic, Tanja; Kamath, Sandip; Kespohl, Sabine; Kleine‐Tebbe, Jörg; Knol, Edward; Knulst, André; Konradsen, Jon R.; Korošec, Peter; Kuehn, Annette; Lack, Gideon; Le, Thuy‐My; Lopata, Andreas; Luengo, Olga; Mäkelä, Mika; Marra, Alessandro Maria; Mills, Clare; Morisset, Martine; Muraro, Antonella; Nowak‐Wegrzyn, Anna; Nugraha, Roni; Ollert, Markus; Palosuo, Kati; Pastorello, Elide Anna; Patil, Sarita Ulhas; Platts‐Mills, Thomas; Pomés, Anna; Poncet, Pascal; Potapova, Ekaterina; Poulsen, Lars K.; Radauer, Christian; Radulovic, Suzana; Raulf, Monika; Rougé, Pierre; Sastre, Joaquin; Sato, Sakura; Scala, Enrico; Schmid, Johannes M.; Schmid‐Grendelmeier, Peter; Schrama, Denise; Sénéchal, Hélène; Traidl‐Hoffmann, Claudia; Valverde‐Monge, Marcela; van Hage, Marianne; van Ree, Ronald; Verhoeckx, Kitty; Vieths, Stefan; Wickman, Magnus; Zakzuk, Josefina; Matricardi, Paolo M.; Hoffmann‐Sommergruber, KarinSince the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE-mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE-mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well-defined, highly pure molecules for component-resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the "EAACI Molecular Allergology User's Guide" (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state-of-the-art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.
- Identification of an elicitin gene cluster in Phytophthora cinnamomi and analysis of the necrotic activity of a purified recombinant beta-cinnamominPublication . Duclos, J.; Aurélio, M.; Graca, J.; Coelho, A. C.; Fauconnier, A.; Jacquet, Alain; Bollen, A.; Cravador, A.; Biemans, R.; Godfroid, EdmondThe oomycetous fungus Phytophthora cinnamoni plays and important role in the necrotic activity observed on feeder root of cork oaks (Quercus suber L.) and eucalyptus (eucalyptus marginata Donn. Ex. Sm.)trees reducing the capacity of these hosts to absorb water and nutrients.
- Structure of beta-cinnamomin, a protein toxic to some plant speciesPublication . Rodrigues, M.; Archer, Margarida; Martel, P.; Jacquet, Alain; Cravador, A.; Carrondo, Maria A.Phytophthora and Pythium species are among the most aggressive plant pathogens, as they invade many economically important crops and forest trees. They secrete large amounts of 10 kDa proteins called elicitins that can act as elicitors of plant defence mechanisms. These proteins may also induce a hypersensitive response (HR) including plant cell necrosis, with different levels of toxicity depending on their pI. Recent studies showed that elicitins function as sterol carrier proteins. The crystallographic structure of the highly necrotic recombinant beta-cinnamomin (beta-CIN) from Phytophthora cinnamomi has been determined at 1.8 Angstrom resolution using the molecular-replacement method. beta-CIN has the same overall structure as beta-cryptogein (beta-CRY), an elicitin secreted by Phytophthora cryptogea, although it shows a different surface electrostatic potential distribution. The protein was expressed in Pichia pastoris and crystallized in the triclinic space group with two monomers in the asymmetric unit. The interface formed by these two monomers resembles that from beta-CRY dimer, although with fewer interactions.