Browsing by Author "Mulero, V."
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- Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approachPublication . Liarte, S.; Chaves-Pozo, E.; Abellán, E.; Meseguer, J.; Mulero, V.; Canario, Adelino V. M.; García-Ayala, A.The role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17 -estradiol (E2) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to identify genes upregulated by E2 (50 ng/ml) in macrophage cultures from gilthead seabream. We isolated 393 upregulated cDNA fragments that led to the identification of 162 candidate estrogen-responsive genes. Functional analyses revealed the presence of several enriched immune processes and molecular pathways. The E2 up-regulation of some immune-relevant genes was further confirmed by real time RT-PCR. Bioinformatics analysis revealed the ability of E2 to orchestrate profound alterations in the macrophage expression profile, especially immune-related processes and pathways. This is the first report on E2- dependent modifications of fish macrophage transcriptome and lends weight to a suggested role for estrogen in the immune system, the possible significance of which is discussed.