Browsing by Author "Neves, A."
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- Evaluation of resistance to Phomopsis amygdali in almondPublication . Cabrita, L.; Neves, A.; Leitão, JoséThe disease caused by the fungus Phomopsis amygdali (Del.) Tuset & Portilla is, today, one of the most important in almond growing, since the main commercial cultivars are susceptible to this pathogen. In Algarve (Portugal), the disease has been causing severe damage to introduced foreign commercial cultivars while traditional cultivars are not significantly affected. Field observations have led to the identification of 'Barrinho Grado' as a local cultivar that has shown non-susceptible behaviour in a heavily infected orchard. Inoculation tests with Phomopsis amygdali isolates were performed on young trees obtained from open-pollinated seeds of 'Barrinho Grado' (resistant) and 'Ferragnes' (susceptible). The 'Barrinho Grado' progeny showed higher resistance to the disease than the population descended from 'Ferragnes'. In order to study the inheritance of resistance to this fungus several controlled crosses were performed involving susceptible and resistant cultivars and the respective progeny plants were inoculated with fungus isolates in order to ascertain their phenotype. Moreover, to evaluate genetic variability of parents involved in the crosses, preliminary studies using isozymes, microsatellites, RAPD and ISSR markers were performed. Genetic similarities between these cultivars were established and suitable molecular markers for genomic mapping were selected. Hybrid populations will be used to construct genetic maps and to identify molecular markers closely linked to the resistance gene(s).
- Prevalence and clinical characteristics of late Onset Axial Spondyloarthritis: Results from a multicentre nationwide studyPublication . Rocha, Margarida Lucas; Torres, R.; Ramiro, S.; Castro, A. M.; Neves, A.; Martins, A.; Chícharo, A. T.; Mendes, B.; Matos, C. O.; Soares, C.; Oliveira, C. P.; Parente, H.; Gomes, J. A. M.; Luís, M.; Santos, M.; Couto, M.; Bernardes, M.; Valente, P.; Costa, R.; Sousa, S.; Branco, J.; Pimentel-Santos, F.; Sepriano, A.Axial spondyloarthritis (axSpA) typically starts before the fourth decade oflife. Consistent with that, the Assessment of SpondyloArthritis international Society (ASAS)classification criteria for axSpA should be applied only in patients with chronic back pain startingbefore 45 years of age. It has, however, been suggested that axSpA can sometimes start later in lifewith a distinctive phenotype, the so-called ‘late onset axSpA’ (lo-axSpA). There is, nevertheless, onlylimited data in support of the existence of such phenotype. We aimed to evaluate the occurrence oflo-axSpA and if these patients differ from those with early onset axSpA (eo-axSpA).
- Zygoparity and sex steroid hormone profiles in bluemouth Helicolenus dactylopterusPublication . Sequeira, V.; Couto, Elsa; Neves, A.; Vieira, A. R.; Paiva, R. B.; Canario, A.V.M.; Gordo, L. S.Two hundred and seven individuals (103 females and 104 males) of bluemouth Helicolenus dactylopterus (Scorpaeniformes, Sebastidae), a commercially important deep-water species with an unusual reproductive strategy, from the eastern Atlantic Ocean ranging from 139 to 375 cm total length (L-T) were analysed from September 2011 to October 2012. The analysis included gonad maturity phases and blood-plasma levels of oestradiol-17 (E-2), 11-ketotestosterone (11-KT) and 17,20-dihydroxypregn-4-en-3-one (17,20-P). Results confirmed the existence of an annual reproductive cycle with asynchrony between females and males and a spawning season from January to May. A pronounced peak in 17,20-P in October for both sexes was associated with possible mating behaviour and recent copula. Levels of E-2 increased preceding the elevation of the gonado-somatic index during ovarian growth and were lower during regression and regeneration. The frequency distribution of oocyte-embryonic stages and variation of hormone levels suggest the existence of daily rhythms. Fertilization was detected between 2000-0000 and 0800-1200 h and spawning took place throughout the day peaking between 2000 and 0000 h. The cyclic pattern of sex steroids and ovarian recruitment provides a new insight into the reproductive strategy of this species.