Browsing by Author "Psenicka, Martin"
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- Biology of teleost primordial germ cells (PGCs) and spermatogonia: Biotechnological applicationsPublication . Robles, Vanesa; F RIESCO, MARTA; Psenicka, Martin; Saito, Taiju; Valcarce, David G.; Cabrita, Elsa; Herraez, PazThis review provides a general view on teleost germline development using primordial germ cells (PGCs) or spermatogonia (SG), highlighting recent progress in research on these two cell types in teleost fishes. Due to the interest of these cells for gene banking purposes, this chapter also reviews the available protocols for cryopreservation and the techniques that have been employed to assess cellular and molecular status after the process. Protocols for in vitro culture and the possibility of generating PGCs in vitro from non-committed embryonic cells are also discussed. Furthermore, the potential of these cells in surrogate production is presented, analyzing the transplantation and xenotransplantation experiments performed in fish. (C) 2016 Elsevier B.V. All rights reserved.
- Molecular basis of spermatogenesis and sperm qualityPublication . Robles, Vanesa; Herraez, Paz; Labbe, Catherine; Cabrita, Elsa; Psenicka, Martin; Valcarce, David G.; F RIESCO, MARTASpermatozoan quality can be evaluated in different ways, here we focus on the analysis of DNA, RNA and epigenetic status of germ cells. These characterizations also can be the bases for explaining sperm quality at other levels, so we will see how some of these molecules could affect other sperm quality markers. Moreover, we consider the possibility of using some of these molecules as predictors of sperm quality in terms of the ability to produce healthy offspring. The relevant effect of different types of RNA molecules in germ line specification and spermatogenesis and the importance of germ cell DNA integrity and a proper epigenetic pattern will be also discussed. Although most studies at this level have been performed in mammals, some information is available for fish; these recent discoveries in fish models are included. We provide a general overview on how these molecules could have a deep influence in the final sperm quality.(C) 2016 Elsevier Inc. All rights reserved.