Browsing by Author "Ramos, Ana"
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- A ciência-cidadã ao serviço da mudança: o exemplo do projeto A Minha PraiaPublication . Gouveia, Licínia; Lourenço, Carla R.; Afonso, João; Freitas, Ricardo; Veiga-Pires, C.; Reis, Emanuel; Rodrigues, Miguel; Gomes, Tiago; Rodrigues, Luís; Mira, Sara; Ferreira, Hélder; Pinto, Andreia; Vaz, Paula; Drago, Teresa; Soares, Isabel; Ramos, AnaVencedor da 1ª edição do Orçamento Participativo Portugal (2017), A Minha Praia é um projeto de monitorização ambiental que pretende sensibilizar para a conservação do meio marinho e estimular hábitos de consumo responsáveis, através de ações de ciência cidadã e civismo ambiental.
- A ciência-cidadã ao serviço da mudança: o exemplo do projeto A Minha PraiaPublication . Gouveia, Licínia; Lourenço, Carla; Afonso, João; Freitas, Ricardo; Veiga-Pires, C.; Reis, Emanuel; Rodrigues, Miguel; Gomes, Tiago; Rodrigues, Luís; Mira, Sara; Ferreira, Hélder; Pinto, Andreia; Vaz, Paula; Drago, Teresa; Soares, Isabel; Ramos, AnaVencedor da 1ª edição do Orçamento Participativo Portugal (2017), A Minha Praia é um projeto de monitorização ambiental que pretende sensibilizar para a conservação do meio marinho e estimular hábitos de consumo responsáveis, através de ações de ciência cidadã e civismo ambiental.
- Biochemistry and molecular biology in Portugal: an overview of past and current contributionsPublication . Ramos, Ana; Varela, JoãoPortugal, represented by the Portuguese Biochemical Society, has been a long-standing member of the IUBMB and a founding member of FEBS.
- Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strainsPublication . Mendoza, Hector; De la Jara, Adelina; Freijanes, Karen; Carmona, Laura; Ramos, Ana; Duarte, Vanessa; Varela, J.In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye Nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.
- Citizen science as a promoter of environmental civility and Nature conservationPublication . Gouveia, Licínia; Ramos, AnaAção Lixo Marinho! is a Portuguese nation-wide Ocean Literacy awareness project that uses citizen science activities of marine litter monitoring as one of the strategies to draw attention of the participants towards a responsible consumption and production (SDG 12) and the conservation and sustainable use of the oceans, seas and marine resources (SDG 14). Due to conclude in March 2022, this project serves as a kickstart to achieve these goals in the long term, with all 12 entities involved in it contributing to an increased engagement in reducing pollution in the marine environment while promoting circular economy, sustainable consumption and waste reduction across the whole of Portuguese society. Project's Website Project's Facebook Profile
- Genetic implications of phylogeographical patterns in the conservation of the boreal wetland butterfly Colias palaeno (Pieridae)Publication . Kramp, Katja; Cizek, Oldrich; Madeira, Pedro M.; Ramos, Ana; Konvicka, Martin; Castilho, Rita; Schmitt, ThomasThe boreo-montane wetland butterfly species Colias palaeno has a European distribution from the Alps to northern Fennoscandia. Within its European range, the species' populations have shrunk dramatically in recent historical times. Therefore, detailed baseline knowledge of the genetic makeup of the species is pivotal in planning potential conservation strategies. We collected 523 individuals from 21 populations across the entire European range and analyzed nuclear (20 allozyme loci) and mitochondrial (600bp of the cytochrome c oxidase subunit I gene) genetic markers. The markers revealed contrasting levels of genetic diversity and divergence: higher in allozymes and lower in mitochondrial sequences. Five main groups were identified by allozymes: Alps, two Czech groups, Baltic countries, Fennoscandia, and Poland. The haplotype mitochondrial network indicates a recent range expansion. The most parsimonious interpretation for our results is the existence of a continuous Wurm glacial distribution in Central Europe, with secondary disjunction during the Last Glacial Maximum into a south-western and a north-eastern fragment and subsequent moderate differentiation. Both groups present signs of postglacial intermixing in the Czech Republic. However, even a complete extinction in this region would not considerably affect the species' genetic basis, as long as the source populations in the Alps and in northern Europe, comprising the most relevant evolutionary units for conservation, are surviving.
- Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene β-cyclasePublication . Ramos, Ana; Coesel, Sacha; Marques, Ana; Rodrigues, Marta; Baumgartner, Alexandra; Noronha, João; Rauter, Amélia; Brenig, Bertram; Varela, J.The halotolerant green alga Dunaliella salina accumulates large amounts of β-carotene when exposed to various stress conditions. Although several studies concerning accumulation and biotechnological production of β-carotene have been published, the molecular basis and regulation of the genes involved in carotenoid biosynthesis in D. salina are still poorly known. In this paper, we report the isolation and regulation of the lycopene β-cyclase (Lcy-β) gene by abiotic stress. The function of this gene was determined by heterologous genetic complementation in E. coli. Gene expression and physiological analyses revealed that D. salina Lcy-β steady-state transcript and carotenoid levels were up-regulated in response to all stress conditions tested (salt, light and nutrient depletion). The results presented here suggest that nutrient availability is a key factor influencing carotenogenesis as well as carotenoid biosynthesis-related gene expression in D. salina.
- Microsatellite markers for the Arctic copepod Calanus glacialis and cross-amplification with C. finmarchicusPublication . Weydmann, Agata; Coelho, Nelson; Ramos, Ana; Serrao, Ester A.; Pearson, GarethCalanus glacialis is a major component of Arctic zooplankton and a keystone species in Arctic marine ecosystems. Due to the observed climate warming, its numbers are being reduced to the advantage of a sibling Atlantic species Calanus finmarchicus. We developed and characterized the first set of microsatellite markers in this species to investigate its population genetic structure and dispersal capabilities. Nine polymorphic loci displayed an average of 7.3 alleles (range between 2 and 13) and the levels of expected heterozygosity ranged from 0.039 to 0.806. These provide a valuable tool to understand present connectivity patterns across Arctic regions, look for signatures of past climate effects and predict the response to future climate-driven environmental changes. Additionally, due to the cross-amplification with C. finmarchicus, the markers can be used to discriminate between these sibling species.
- Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salinaPublication . Ramos, Ana; Marques, A. R.; Rodrigues, M.; Henriques, Nuno; Baumgartner, Alexandra; Castilho, Rita; Brenig, Bertram; Varela, J.In green algae,the final step of the plastidial methylerythritol phosphate (MEP) pathway is catalyzed by 4-hydroxy-3-methylbut-2-enyldiphosphate reductase(HDR; EC: 1.17.1.2),an enzyme proposed to play a keyrole in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 1959- bp Dunaliella salina HDR (DsHDR) cDNA encoding a deduced polypeptide of 474 amino acid residues. Phylogenetic analysis implied a cyanobacterial origin for plant and algal HDR genes. Steady-state DsHDR transcript levels were higher in D. salina cells submitted to nutritional depletion, high salt and/or high light, suggesting that DsHDR may respond to the same environmental cues as genes involved in carotenoid biosynthesis.
- Molecular basis of isoprenoid biosynthesis in Dunaliella salinaPublication . Ramos, Ana; Varela, J.