Browsing by Author "Rodriguez, Covadonga"
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- Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlingsPublication . Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Tocher, Douglas R.; Andrade, Jose; Sykes, António; Rodriguez, CovadongaThe objective of the present study was to characterise the fatty acid (FA) profiles of the major phospholipids, of Octopus vulgaris and Sepia officinalis hatchlings, namely phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylethanolamine (PE); and to evaluate the capability of both cephalopod species on dietary phospholipid remodelling. Thus, O. vulgaris and S. officinalis hatchlings were in vivo incubated with 0.3 mu M of L-(alpha)-1-palmitoyl-2-[1-C-14]arachidonyl-PC or L-(alpha)-1-palmitoy1-2-[1-C-14]arachidonyl-PE. Octopus and cuttlefish hatchlings phospholipids showed a characteristic FA profiles with PC presenting high contents of 16:0 and 22:6n-3 (DHA); PS having high 18:0, DHA and 20:5n-3 (EPA); PI a high content of saturated FA; and PE showing high contents of DHA and EPA. Interestingly, the highest content of 20:4n-6 (ARA) was found in PE rather than PI. Irrespective of the phospholipid in which [1-C-14]ARA was initially bound (either PC or PE), the esterification pattern of [1-C-14]ARA in octopus lipids was similar to that found in their tissues with high esterification of this FA into PE. In contrast, in cuttlefish hatchlings [1-C-14]ARA was mainly recovered in the same phospholipid that was provided. These results showed a characteristic FA profiles in the major phospholipids of the two species, as well as a contrasting capability to remodel dietary phospholipids, which may suggest a difference in phospholipase activities. (C) 2016 Elsevier Inc. All rights reserved.
- Effect of of Artemia inherent fatty acid metabolism on the bioavailability of essential fatty acids for Octopus vulgaris paralarvae developmentPublication . B Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Garrido, Diego; Andrade, José Pedro; Sykes, Antonio V.; Rodriguez, CovadongaThe aim of the present study was to determine the effect of Artemia metanauplii endogenous fatty acid (FA) metabolism in the actual availability of dietary essential fatty acids (EFA) for Octopus vulgaris paralarvae development. To this end, both Artemia metanauplii inherent radiolabelled FA metabolism and the Octopus vulgaris paralarvae FA metabolism, after being fed with Artemia metanauplii incubated with radiolabelled. FAs, were determined. Metanauplii were incubated in tissue culture plates during 12 h, with 0.3 mu M of a [1-C-14]FA, including either 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA), added individually to separate wells as their potassium salts bound to bovine serum albumin. A total of 3600 one-day-old paralarvae were reared up to 24 h in order to ensure the ingestion of a minimum amount of the labelled Artemia. Paralarvae rearing was performed in 4 L cylinder conical tanks at a density of 25 paralarvae L-1. Labelled Artemia metanauplii were added to each paralarvae rearing tank according to a specific [1-C-14]FA treatment. Artemia in vivo metabolism results showed a preferential catabolism of DHA, which was translated into (1) the lower incorporation of this FA into Artemia total lipids (TL)