Percorrer por autor "Shehzad, Adeeb"
A mostrar 1 - 3 de 3
Resultados por página
Opções de ordenação
- Camptothecin-loaded mesoporous silica nanoparticles functionalized with CpG oligodeoxynucleotide as a new approach for skin cancer treatmentPublication . Qureshi, Munibah; Viegas, Cláudia; Duarte, Sofia O.D.; Girardi, Michael; Shehzad, Adeeb; Fonte, PedroThe therapeutic efficacy of camptothecin (CPT), a potent antitumor alkaloid, is hindered by its hydrophobic nature and instability, limiting its clinical use in treating cutaneous squamous cell carcinoma (SCC). This study introduces a novel nano drug delivery system (NDDS) utilizing functionalized mesoporous silica nanoparticles (FMSNs) for efficient CPT delivery. The FMSNs were loaded with CPT and subsequently coated with chitosan (CS) for enhanced stability and bioadhesion. Importantly, CpG oligodeoxynucleotide (CpG ODN) was attached onto the CS-coated FMSNs to leverage the immunostimulatory properties of CpG ODN, augmenting the chemotherapy's efficacy. The final formulation FMSN-CPT-CS-CpG displayed an average size of 241 nm and PDI of 0.316 with an encapsulation efficiency of 95 %. Comprehensive in vitro and in vivo analyses, including B16F10 cells and DMBA/TPA-induced SCC murine model, demonstrated that the FMSN-CPT-CS-CpG formulation significantly enhanced cytotoxicity against B16F10 cells and induced complete regression in 40 % of the in vivo subjects, surpassing the efficacy of standard CPT and FMSN-CPT treatments. This study highlights the potential of combining chemotherapeutic and immunotherapeutic agents in an NDDS for targeted, efficient skin cancer treatment.
- Nanomedicine in oncology: diagnostic breakthroughs and therapeutic FrontiersPublication . Shehzad, Adeeb; Alves, Júlia; Ul-Islam, Mazhar; Saidi, Abdullah Khamis Al; Duarte, Sofia O. D.; Khan, Mohammad Sherjeel Javed; Fonte, PedroNanomedicine is a multidisciplinary field, offering significant promises for cancer detection and therapy. Nanoparticles (NPs), nanoprobes and nanobiosensors can be tailored to achieve highly sensitive tumor detection by contrast imaging techniques. The application of directed drug delivery for cancer therapies can be achieved via the formulation and tailoring of drug-loaded nanocarriers. NPs have been employed as carrier to transport drugs or biological molecules to tumor tissues via active or passive mechanisms, consequently improving treatment outcomes and minimize harmful effects. However, nanomedicine translation has been hindered by augmented permeability and retention and ICI of the TME, limiting improvement and potential outcomes of patients. TME, consisting of cancerous cells, CAFs or TAFs, specific immune cells, and the stroma, performs a crucial part in contributing to cancer resistance to nanotherapy. This review summarizes nanotechnology application in the identification and treatment of cancers by exploring pathophysiological features, mechanisms and limitation of nanomedicine in cancer.
- Synergistic effect of aloe vera hydrogels with imatinib for pH-responsive drug release in breast cancer treatmentPublication . Khan, Aroob Hasan; Shehzad, Adeeb; Pirela, Paola; Atalaia, Mariana; Ruivinho, Beatriz Lourenço; Rashan, Luay; Miran, Waheed; Duarte, Sofia O. D.; Fonte, PedroImatinib (IM) efficacy as a cancer drug is limited by pharmacokinetic drug resistance developed during systemic circulation before reaching the target site.Hydrogels have attracted attention because of their characteristic physiochemical and biochemical properties, flexibility, and the ability to release drugs directly at target sites causing cancer mitigation. The current study aims at developing Aloe Vera (AV) hydrogels for the efficient and targeted delivery of IM into cancer cells and studying its synergistic effect. Incorporating Aloe Vera into the previously studied Sodium Alginate (SA)/PolyVinyl Alcohol (PVA) hydrogels and loading with IM is expected to reach a pH-responsive release efficiency, enhanced biochemical properties and increased cancer cell cytotoxicity. The hydrogels, SA/PVA and SA/PVA/AV were characterized (FT-IR, SEM) and investigated for their physiochemical properties. The presence of AV and IM were confirmed by the increase in the intensity of band from 3000 to 3500 cm-1, while an increase in the pore size was observed upon the loading of IM. The final formulation, SA/PVA/AV hydrogels displayed increased pore size which leveraged their swelling, degradation, encapsulation, and release properties by 400%, 100%, 56%, and 94%, respectively. The in vitro analysis on breast cancer cells showed that the SA/PVA/AV hydrogels loaded with IM worked synergistically to significantly reduce the cancer cell viability to 40%, surpassing the efficacy of the SA/PVA/AV hydrogel and IM treatments alone. This study highlights the promising potential for the use of AV in the development of a drug delivery system (DDS) for targeting and improving therapeutic outcomes in cancer treatment.