Percorrer por autor "Zengin, Gökhan"
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- Antioxidant activity, enzyme inhibition, photoprotection, cytotoxicity, and phytochemical profiling of sea lavender (Limonium algarvense Erben) seed extracts for dermo-cosmetic usePublication . Lescano, Leonardo; Cziáky, Zoltán; Kurt-Celep, İnci; Zengin, Gökhan; Fernandes, Eliana; Trentin, Riccardo; Guerreiro Pereira, Catarina Alexandra; Custódio, Luísa; Rodrigues, Maria JoãoDespite sea lavender being a medicinal species, research on its seeds' biological properties and chemical composition is unexplored. Thus, this study evaluated the effect of different extraction solvents on the biological activities and chemical profile of greenhouse-cultivated sea lavender seeds, aiming at their potential use as a dermo-cosmetic ingredient. Therefore, ethyl acetate, acetone, ethanol, and water extracts were examined for their antioxidant activity, enzyme inhibition, photoprotection, and cytotoxicity, followed by phytochemical analysis through spectrophotometric methods, further detailed by Ultrahigh-Performance Liquid Chromatography Coupled with Electrospray Ionization Mass/Mass Spectrometry (UHPLC-Esi-MS/MS).ResultsThe water extract demonstrated significant antioxidant activity, evidenced by low half maximal effective concentration (EC50) values in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing iron and chelating copper (296, 478, 230 and 678 mu g/mL, respectively). The ethanol extract was more effective in inhibiting cosmetic-related enzymes, particularly elastase and hyaluronidase (2.18 and 3.21 mu g/mL, respectively). The water and acetone extracts had the highest sun protection factors (23.2 and 18.9, respectively). All the extracts had nil to weak cytotoxicity (70-120% cell viability) towards mammalian cell lines. The water extract had the highest phenolics and condensed tannins (115 and 78.30 mg/g extract, respectively), while the ethanol contained the most flavonoids (62.73 mg/g extract). UHPLC-ESI-MS/MS analysis identified ethyl gallate, myricetin, rutin, and quercetin as major components of the ethanol extract, whereas myricetin-O-rutinoside isomers are predominant in the water extract.ConclusionsThese findings highlight the potential of greenhouse-cultivated sea lavender seeds as potential dermo-cosmetic ingredients, with ethanol and water extracts demonstrating superior biological activities and chemical profiles, significantly contributing to general skin health and protection.
- Delving into the phytochemical constituents and biological activities of Scorzonera coriacea extracts: new perspectives from in vitro and in silico studiesPublication . Zengin, Gökhan; Ak, Gunes; Yagi, Sakina; Cetiz, Mehmet Veysi; Tutuş, Ramazan; Rodrigues, Maria João; Fernandes, Eliana; Custódio, Luísa; Yildiztugay, Evren; Aly, Shaza H.; Eldahshan, Omayma A.; Singab, Abdel Nasser B .The current study was designed to investigate the chemical composition, antioxidant, enzyme inhibitory, and cytotoxic activities of Scorzonera coriacea A.Duran & Aksoy. Both organs were rich in total phenolic content, with the highest content recorded from the 70% EtOH (48.41 mg GAE/g) and aqueous (47.11 mg GAE/g) extracts of the roots. All aerial parts extracts accumulated higher total flavonoid content than their respective roots extracts, with the highest amount found in their EtOH extract (36.44 mg RE/g). Chemical analysis revealed the presence of 86 compounds belonging to organic acids, phenolic acids, flavonoids, coumarins, anthocyanins, terpenes, saponins, and fatty acids and their derivatives, with the aerial parts accumulating the highest number. The roots displayed the strongest antiradical and ion-reducing capacities. EtOH extract of both organs recorded the highest acetylcholinesterase activity (2.77 and 3.02 mg GALAE/g; p ≥ 0.05), while that of the root showed the best butyrylcholinesterase activity (3.49mg GALAE/g) and that of the aerial parts the best tyrosinase inhibitory (59.07 mg KAE/g). EtOAc of the root exhibited the best cytotoxicity towards theHepG2 cell line (cell viability = 29.30%), butwas also toxic towards HEK293 cells (cell viability = 11.72%). In silico screening supported these findings by identifying multiple strong ligand–protein interactions. Molecular dynamics simulations further confirmed the structural stability of selected complexes. In silico profiling docked 26 phytochemicals against 14 therapeutic targets, generating 364 complexes, of which 62% showed ΔG ≤ −7.0 kcal⋅mol−1. Binding energies ranged from−1.4 to −10.7 kcal⋅mol−1, with PD-1–Eriodictyol-7-O-neohesperidoside the best. For metabolic enzymes, Eriodictyol-7-O-neohesperidoside yielded the top α-amylase score and Diosmetin-7-O-glucoside the top α-glucosidase score, while several flavonoids bound AChE/BChE strongly; in contrast, tyrosinase displayed poor affinity overall. 100-ns MD simulations on five top complexes indicated stable behavior for C1 and C4, whereas C2/C3/C5 showed loosening interactions over time.These findings showed that S. coriacea could be a promising source of bioactive compounds with potential therapeutic applications.
- A multidirectional study on chemical fingerprints and biological activities of three Cistus extracts (C. creticus, C. laurifolius, and C. salviifolius) with ethnomedicinal usesPublication . Yagi, Sakina; Zengin, Gökhan; SELVİ, Selami; AK, Güneş; CZIÁKY, Zoltán; Jekő, József; Rodrigues, Maria João; Custódio, Luísa; Venanzoni, Roberto; Flores, Giancarlo Angeles; Cusumano, Gaia; ANGELINI, PaolaHumans have used medicinal plants to treat various diseases for thousands of years. Cistus species are also widely used in traditional medicine and have various medicinal applications; therefore, they deserve more in-depth research. The present study evaluated the chemical profile, antioxidant, enzyme inhibition, and cytotoxic properties of the twigs and leaves of C. creticus L., C. laurifolius L., and C. salviifolius L. grown in Türkiye. The methanolic extracts of the three species were rich in phenolics, mainly flavonoids. Exerted potent antioxidant activity with a methanolic extract from the leaves of C. salviifolius displayed the highest total phenolic (97.08-mg gallic acid equivalent/g) and flavonoid (49.60-mg rutin equivalent/g) contents, as well as antiradical (2,2-diphenyl-1-picrylhydrazyl) assay = 612.11 mg TE (trolox equivalent)/g; (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) = 804.66 mg TE/g), reducing ions (cupric reducing antioxidant capacity) = 690.54 mg TE/g; ferric reducing antioxidant power = 459.34 mg TE/g), and chelating (15.58 mg EDTAE/g) properties. It also revealed the best amylase and glucosidase inhibitory activity. Extracts from the twigs of the three Cistus species, except the leaves of C. salviifolius and C. laurifolius, displayed comparable acetylcholinesterase inhibitory activity (2.48–2.57 mg galanthamine equivalent (GALAE)/g). The twig of C. laurifolius also exerted the best antibutyrylcholinesterase (10.50 mg GALAE/g) and antityrosinase (73.15 mg kojic acid equivalent/g) activities. C. creticus leaves revealed toxicity toward the RAW cell line (cell viability reduced to 68.8%) and were not toxic to normal cells (S17). In conclusion, these three Cistus species were shown to be a rich source of bioactive compounds with the potential for future applications in the food, pharmaceutical, and cosmetic industries.
- Salt-tolerant plants as sources of Antiparasitic Agents for human use: A comprehensive reviewPublication . Rodrigues, Maria João; Pereira, Catarina Guerreiro; Oliveira, Marta; Zengin, Gökhan; L, CustódioParasitic diseases, especially those caused by protozoans and helminths, such as malaria, trypanosomiasis, leishmaniasis, Chagas disease, schistosomiasis, onchocerciasis, and lymphatic filariasis, are the cause of millions of morbidities and deaths every year, mainly in tropical regions. Nature has always provided valuable antiparasitic agents, and efforts targeting the identification of antiparasitic drugs from plants have mainly focused on glycophytes. However, salt-tolerant plants (halophytes) have lately attracted the interest of the scientific community due to their medicinal assets, which include antiparasitic properties. This review paper gathers the most relevant information on antiparasitic properties of halophyte plants, targeting human uses. It includes an introduction section containing a summary of some of the most pertinent characteristics of halophytes, followed by information regarding the ethnomedicinal uses of several species towards human parasitic diseases. Then, information is provided related to the antiprotozoal and anthelmintic properties of halophytes, determined by in vitro and in vivo methods, and with the bioactive metabolites that may be related to such properties. Finally, a conclusion section is presented, addressing perspectives for the sustainable exploitation of selected species.