Browsing by Author "Zhu, You-Ting"
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- The mussel larvae microbiome changes in response to a temperature risePublication . Zhu, You-Ting; Liang, Xiao; Liu, Tian-Tian; Power, Deborah Mary; Li, Yi-Feng; Yang, Jin-LongOcean warming caused by global climate change influences the function, diversity, and community dynamics of commensal microorganisms, including the hemolymph and the gut microbiota in mussels. However, the microbiota in hard-shelled mussel (Mytilus coruscus) larvae and the effect of temperature on the microbial community structure have yet to be studied. Herein, we investigated the core microbiota of M. coruscus larvae and the impact of acute (4 h) and gradual (4 days) exposure to a rise in seawater temperature from 21 to 25 degrees C. Eleven core genera were identified in M. coruscus larvae by 16S rDNA gene sequencing: Alteromonas, Brevundimonas, Delftia, Microbacterium, Neptuniibacter, Neptunomonas, Pseudoalteromonas, Rhodococcus, Stenotrophomonas, Tenacibaculum, and Thalassotalea. The microbiota of larvae in the short exposure treatment was similar to the control. However, the abundance of Delftia, Neptunomonas, Pseudoalteromonadaceae, Rhodococcus, and Stenotrophomonas decreased significantly in the long-exposure larvae. In contrast, at the genus level, the abundance of Tenacibaculum increased significantly. Diversity and multivariate analyses confirmed that the microbiota patterns were linked to seawater warming over the long term. Microbiota diversity did not change significantly, regardless of whether the seawater temperature increased quickly or slowly; however, we observed a significant increase in the microbiota species abundance at higher temperatures. Among the altered bacterial genera, Delftia, Neptunomonas, and Rhodococcus function in the degradation of organic compounds; Pseudoalteromonas is closely associated with mussel attachment and metamorphosis, and Tenacibaculum is an opportunistic pathogen that can cause marine mollusk death. The results suggest that marine heat waves caused by climate change may reduce the ability of symbiotic bacteria to degrade environmental toxins, will affect mussel larvae metamorphosis, and increase the abundance of opportunistic pathogens, thereby increasing the risk of disease and death of mussel larvae.
- Thyroid hormone receptor: a new player in epinephrine-induced larval metamorphosis of the hard-shelled musselPublication . Li, Yi-Feng; Cheng, Yu-Lan; Chen, Ke; Cheng, Zhi-Yang; Zhu, Xin; Cardoso, João; Liang, Xiao; Zhu, You-Ting; Power, Deborah; Yang, Jin-LongMany marine invertebrate larvae undergo a dramatic morphological and physiological transition from a planktonic larva to a benthic juvenile. The mechanisms of this metamorphosis in bivalves are mainly unknown. The recent identification in bivalves of a thyroid hormone receptor (TR) gene raises the possibility that as occurs in vertebrate metamorphosis, TRs regulate this developmental process. An evolutionary study of TR receptors revealed they are ubiquitous in the molluscs. Knock-down of the TR gene in pediveliger larvae of the hard-shelled mussel, Mytilus coruscus (Mc), using electroporation of siRNA significantly (p < 0.01) reduced TR gene expression. TR gene knock-down decreased pediveliger larval metamorphosis by 54% and was associated with a significant (p < 0.01) reduction in viability compared to control larvae. The TR in the hard-shelled mussel appears to be an essential regulatory factor for the successful epinephrine-induced metamorphosis of the pediveliger larvae to post-larvae. It is hypothesised that the knock-down of TR by siRNA transfection affects the "competence" of pediveliger larvae for the metamorphic transition by reducing their ability to respond to the inducer. The involvement of TR in the epinephrine-induced metamorphosis of a mollusc, the hard-shelled mussel, suggests the role of TR in this process probably emerged early during evolution.
- Two toll-like receptors identified in the mantle of Mytilus coruscus are abundant in haemocytesPublication . Li, Yi-Feng; Liu, Yu-Zhu; Chen, Yan-Wen; Chen, Ke; Batista, Frederico; Cardoso, João CR; Chen, Yu-Ru; Peng, Li-Hua; Zhang, Ya; Zhu, You-Ting; Liang, Xiao; Power, Deborah; Yang, Jin-LongToll-like receptors (TLRs) are a large family of pattern recognition receptors (PREs) that play a critical role in innate immunity. TLRs are activated when they recognize microbial associated molecular patterns (MAMPs) of bacteria, viruses, or fungus. In the present study, two TLRs were isolated from the mantle of the hard-shelled mussel (Mytilus coruscus) and designated McTLR2 and McTLR3 based on their sequence similarity and phylogenetic clustering with Crassostrea gigas, CgiTLR2 and CgiTLR3, respectively. Quantitative RT-PCR analysis demonstrated that McTLR2 and McTLR3 were constitutively expressed in many tissues but at low abundance.