Browsing by Issue Date, starting with "2007-12"
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- Gestão estratégica da Região de Lisboa e Vale do Tejo - Relatório 4Publication . Sousa, Vanessa Duarte deO projecto “Gestão Estratégica da RLVT”, desenvolvido pela Comissão de Coordenação e Desenvolvimento Regional de Lisboa e Vale do Tejo, constitui uma experiência inovadora no campo da aplicação prática da monitorização da gestão estratégica de uma região. Estes seis anos de trabalho e de vida do projecto, com a edição de vários documentos e relatórios que em muito têm servido para melhorar os processos de decisão, a definição de estratégias e de prioridades para o desenvolvimento da nossa Região – contribuíram decisivamente para uma nova fase de reflexão e de apoio à elaboração da “Estratégia Regional, Lisboa 2020” e ao Programa Operacional Regional de Lisboa (2007-2013). O “Relatório 4”, que segue a lógica dos anteriores (Relatório 0, 1, 2 e 3), e se baseia na actualização estatística dos indicadores que sustentam as principais dimensões de análise seleccionadas em cada um dos domínios de monitorização definidos – Território, Pessoas e Organizações - apresenta dados até 2004/2006.
- A construção das identidades urbanas como factor de repulsão dos territórios ruraisPublication . Sousa, Vanessa Duarte deO texto analisa a relação existente entre a construção identitária e a mudança em curso nos espaços rurais. Justifica-se o abandono dos jovens de um espaço rural- Cachopo pelas socializações crescentes nos espaços urbanos. Conclui-se que o contacto continuado com os espaços urbanos conduz a uma multiplicidade de referências identitárias onde o modelo de desenvolvimento que é preconizado nestes espaços é aquele que mais seduz os jovens ao nível dos seus projectos de vida.
- Insight into stability of CotA laccase from the spore coat of Bacillus subtilisPublication . EP, Melo; Fernandes, A. T.; Durao, P.; Martins, L. O.The axial ligand of the catalytic mononuclear T1 copper site (Met(502)) of the CotA laccase was replaced by a leucine or phenylalanine residue to increase the redox potential of the enzyme. These mutations led to an increase in the redox potential by approx. 100 mV relative to the wild-type enzyme but the catalytic constant k(cat) in the mutant enzymes was severely compromised. This decrease in the catalytic efficiency was unexpected as the X-ray analysis of mutants has shown that replacement of methionine ligand did not lead to major structural changes in the geometry of the T1 Centre or in the overall fold of the enzyme. However, the mutations have a profound impact on the thermodynamic stability of the enzyme. The fold of the enzyme has become unstable especially with the introduction of the larger phenylalanine residue and this instability should be related to the decrease in the catalytic efficiency. The instability of the fold for the mutant proteins resulted in the accumulation of an intermediate state, partly unfolded, in-between native and unfolded states. Quenching of tryptophan fluorescence by acrylamide has further revealed that the intermediate state is partly unfolded.
- Chemical and microbial changes during the natural fermentation of strawberry tree (Arbutus unedo L.) fruitsPublication . Cavaco, Teresa; Longuinho, Carla; Quintas, Célia; Carvalho, Isabel Saraiva deAguardente de medronho is the name given in Portugal to a spirit made from the fermented fruit of Arbutus unedo (strawberry tree), a plant grown in the Mediterranean region. In order to gain a better understanding of the fermentation process, as it is performed in the farms, a natural fermentation with wild microbiota was carried out during 36 days, and some physicochemical and microbiological parameters were studied. The microbial parameters analyzed were total viable, lactic and acetic acids bacteria, and yeast counts. The physicochemical parameters monitored were sugars, minerals, ethanol, organic acids and pH. Yeasts were the main responsible for the fermentation of the fruits, as the lactic and acetic acids bacteria are absent. As the fermentation progressed, the sugars increased during the first 2 days and gradually decreased along the fermentation period. Maintaining the good quality of the product could contribute to the preservation and valorization of traditional resources that are of great importance to prevent their disappearance.
- The ecological role of overwintering fish in the food web of the Culbin Sands lagoon ecosystem, NE Scotland: identifying major trophic links and testing effects of the fish Pomatoschistus microps (Pallas) on benthic invertebratesPublication . Mendonca, Vanda; Raffaelli, David; Boyle, Peter; Emes, ChasThe Culbin Sands lagoon ecosystem in NE Scotland was studied during a three-year period (1994-1996) to identify the major trophic links from benthic invertebrates to epibenthic predators, and to assess impacts of overwintering fish on their prey communities. Every 2-4 weeks, samples of mobile fauna were collected to study their diets. The major trophic links identified between benthic invertebrates and epibenthic predators were from benthic invertebrates to the shrimp Crangon crangon, and to the common goby Pomatoschistus microps and the plaice Pleuronectes platessa. The energy flow from benthic invertebrates to overwintering fish was estimated at 133 kJ m(-2)yr(-1). A flow of 10 kJ m(-2)yr(-1) was also observed from eggs and larval stages of the overwintering shrimp Crangon crangon to the overwintering fish. Nevertheless, manipulative field experiments showed no significant impacts of the most abundant overwintering fish Pomatoschistus microps on prey community densities, despite an overall individual ingestion rate of 89 J day(-1).
- Male urine signals social rank in the Mozambique tilapia (Oreochromis mossambicus)Publication . Barata, E. N.; Hubbard, Peter; Almeida, O. G.; Miranda, A.; Canario, Adelino V. M.The urine of freshwater fish species investigated so far acts as a vehicle for reproductive pheromones affecting the behaviour and physiology of the opposite sex. However, the role of urinary pheromones in intra-sexual competition has received less attention. This is particularly relevant in lek-breeding species, such as the Mozambique tilapia (Oreochromis mossambicus), where males establish dominance hierarchies and there is the possibility for chemical communication in the modulation of aggression among males. To investigate whether males use urine during aggressive interactions, we measured urination frequency of dye-injected males during paired interactions between size-matched males. Furthermore, we assessed urinary volume stored in the bladder of males in a stable social hierarchy and the olfactory potency of their urine by recording of the electro-olfactogram. Males released urine in pulses of short duration (about one second) and markedly increased urination frequency during aggressive behaviour, but did not release urine whilst submissive. In the stable hierarchy, subordinate males stored less urine than males of higher social rank; the olfactory potency of the urine was positively correlated with the rank of the male donor. Dominant males store urine and use it as a vehicle for odorants actively released during aggressive disputes. The olfactory potency of the urine is positively correlated with the social status of the male. We suggest that males actively advertise their dominant status through urinary odorants which may act as a 'dominance' pheromone to modulate aggression in rivals, thereby contributing to social stability within the lek.
- Como podemos nós melhorar as relações entre o Japão e PortugalPublication . Aníbal, J.Anualmente, ao longo dos últimos 29 anos, o Ministério dos Negócios Estrangeiros do Japão lança, através das suas Embaixadas nos Países Europeus, um Concurso de Ensaios.