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- Endogenous calcification inhibitors in the prevention of vascular calcification: a consensus statement from the COST action EuroSoftCalcNetPublication . Bäck, Magnus; Aranyi, Tamas; Cancela, M. Leonor; Carracedo, Miguel; Conceição, Natércia; Leftheriotis, Georges; Macrae, Vicky; Martin, Ludovic; Nitschke, Yvonne; Pasch, Andreas; Quaglino, Daniela; Rutsch, Frank; Shanahan, Catherine; Sorribas, Victor; Szeri, Flora; Valdivielso, Pedro; Vanakker, Olivier; Kempf, HervéThe physicochemical deposition of calcium-phosphate in the arterial wall is prevented by calcification inhibitors. Studies in cohorts of patients with rare genetic diseases have shed light on the consequences of loss-of-function mutations for different calcification inhibitors, and genetic targeting of these pathways in mice have generated a clearer picture on the mechanisms involved. For example, generalized arterial calcification of infancy (GACI) is caused by mutations in the enzyme ecto-nucleotide pyrophosphatase/phosphodiesterase-1 (eNPP1), preventing the hydrolysis of ATP into pyrophosphate (PPi). The importance of PPi for inhibiting arterial calcification has been reinforced by the protective effects of PPi in various mouse models displaying ectopic calcifications. Besides PPi, Matrix Gla Protein (MGP) has been shown to be another potent calcification inhibitor as Keutel patients carrying a mutation in the encoding gene or Mgp-deficient mice develop spontaneous calcification of the arterial media. Whereas PPi and MGP represent locally produced calcification inhibitors, also systemic factors contribute to protection against arterial calcification. One such example is Fetuin-A, which is mainly produced in the liver and which forms calciprotein particles (CPPs), inhibiting growth of calcium-phosphate crystals in the blood and thereby preventing their soft tissue deposition. Other calcification inhibitors with potential importance for arterial calcification include osteoprotegerin, osteopontin, and klotho. The aim of the present review is to outline the latest insights into how different calcification inhibitors prevent arterial calcification both under physiological conditions and in the case of disturbed calcium-phosphate balance, and to provide a consensus statement on their potential therapeutic role for arterial calcification.
- Evaluation of macrobenthic community responses to dredging through a multimetric approach: Effective or apparent recovery?Publication . Piló, David; Carvalho, A. N.; Pereira, F.; Coelho, H. E.; Gaspar, MiguelMacrobenthic responses to dredging activities were evaluated in a mesotidal coastal lagoon (Ria Formosa South Portugal). Four areas (2 dredged and 2 non-dredged) were analysed along different dredging situations (pre-dredging, during dredge 1, during dredge 2 and post-dredging). Beyond the evaluation of sediment descriptors and classical ecological indicators (number of species and abundance), the beta diversity component (partitioned into turnover and nestedness) was used to detect dredging effects on macrobenthic communities, together with a multivariate analysis (both quantitative and qualitative) including the ecological status assessment. Results confirmed the limitations of alpha diversity indicators in detecting dredging effects in naturally stressed systems, given the absence of clear patterns regarding number of species, abundance and equitability among areas and dredging situations. Moreover, post-dredging presented a higher number of species compared to pre-dredging, suggesting a positive disequilibrium induced by these activities. In contrast, partitioned beta diversity analysis revealed higher nestedness values at dredged areas, while turnover clearly prevailed at non-dredged ones. Such results suggest an effective impact of dredging on these communities, namely through the decrease of rarer species after sediment removal. This qualitative analysis, based on presence absence data (Sorensen dissimilarity), was complemented by multivariate analysis (SIMPER and PERMANOVA) based on quantitative data (Bray-Curtis dissimilarity), highlighting the importance of adopting both approaches to detect dredging impacts on benthic communities. Lower effectiveness was found for M-AMBI index when evaluating benthic Ecological Status, since the apparent absence of organic matter and sediment composition shifts induced by dredging, together with the noticeable high diversity even during dredging situations, strongly limited the analysis. The multimetric approach here proposed can contribute to detect dredging effects on lagoonal benthic communities by disentangling the responses caused by dredging from those resulting from the system natural dynamics.
- A comparative study of the in vitro enzyme inhibitory and antioxidant activities of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret from Pakistan: New sources of natural products for public health problemsPublication . Baessa, M.; Rodrigues, Maria João; Pereira, Catarina; Santos, T.; Neng, N. da Rosa; Nogueira, J. M. F.; Barreira, Luísa; Varela, J.; Ahmed, H.; Asif, S.; Boukhari, S. A.; Kayani, W. K.; Ahmad, Khawaja Shafique; Zengin, G.; Mollica, A.; Custódio, LuísaInfusions, decoctions and tinctures were prepared from flowers of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret and evaluated for in vitro inhibition of enzymes implicated on the onset of neurological diseases (acetylcholinesterase: AChE and butyrylcholinesterase: BuChE), diabetes (alpha-glucosidase and alpha-amylase), obesity (lipase) and skin hyperpigmentation (tyrosinase). Extracts were also appraised for radical scavenging activity (RSA) on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and for metal chelating activity on copper and iron ions. Samples were evaluated for their total contents in different phenolics groups by spectrophotometric methods, for phenolic profile by high performance liquid chromatography e diode array detection (HPLC-DAD) and for mineral contents by microwave plasma-atomic emission spectrometry (MP-AE). Regarding B. monosperma, the tincture allowed for a moderate inhibition of AChE, the decoction was able to inhibit alpha-glucosidase and no activity was observed towards BuChE, alpha-amylase or lipase. All extracts had a low or moderate inhibition towards tyrosinase, and significant RSA and metal chelating potential. As for S. grandiflora, only the decoction inhibited AChE, none of the extracts was able to inhibit BuChE, all samples inhibited alpha-glucosidase and infusions and decoctions had similar inhibitory properties towards alpha-amylase. None of the extracts was active against lipase, but all were able to inhibit tyrosinase. Extracts had also significant RSA, moderate copper chelation and decoctions had the capacity to chelate iron. The most abundant macroelements in both species were potassium and calcium, while iron was the prevalent microelement, especially in B. monosperma. Both species had significant levels of phenolic compounds, and the main components in decoctions and infusions of B. monosperma were syringic and salicylic acids, while the major compound identified in tinctures was the flavonoid luteolin-7-O-glucoside. In S. grandiflora the most abundant were chlorogenic and neochlorogenic acids and catechin hydrate. Molecular docking studies on the most abundant molecules in S. grandiflora, (+)-catechin, chlorogenic acid and neochlorogenic acid, indicate that these compounds are able to dock to alpha-glucosidase in a similar manner than acarbose. Our results suggest that flowers of both species are a promising source of high value-added compounds with enzyme inhibitory and antioxidant properties. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.
- A cultura da framboesa. Presença de néctar nas flores e sua extração pelas abelhas.Publication . Brito, Tomás de Almeida; Lhérété, Jean-Pierre; Pereira, Jorge; Duarte, AmílcarA cultura da framboesa tem vindo a ganhar uma importância crescente nos últimos 20 anos, sobretudo no Algarve e na Costa Vicentina. Em 2017 as estimativas apontam para 1108 hectares cultivados, representando 17 880 toneladas produzidas a nível nacional (INE,2017), das quais, a maior parte se destinou à exportação.
- Surface-ocean dynamics during eccentricity minima: a comparison between interglacial Marine Isotope Stage (MIS) 1 and MIS 11 on the Iberian MarginPublication . Palumbo, Eliana; Voelker, Antje; Flores, Jose Abel; Amore, Ornella F.Understanding interglacial climate variability is a key issue in the scientific community. Here we compared records from Marine Isotope Stage (MIS) 11 to those from MIS 1 (Holocene) as they are perceived to be possible analogs. Our study on the Iberian Margin, a key area to investigate surface dynamics in the Atlantic Ocean, incorporates coccolithophore assemblage and alkenone data of core MD03-2699 and their statistical analyses. Evaluating similarities between MIS 11 and MIS 1 depends on the way the two MIS are being aligned, i.e. at the deglaciation or based on the precession signal. During the deglaciation of either MIS 12 or MIS 2, the Iberian Margin was affected by abrupt decreases in SST and in coccolithophores' paleoproductivity caused by the arrival of subpolar surface waters. Just prior to the decline, in both the intervals, the Portugal Current affected the studied site, although a possible difference in upwelling strength is here suggested and related to more intense westerlies during the last glacial than the late MIS 12. Similar surface-ocean dynamics occurred at the onset of both MIS 11 and MIS 1 as indicated by the prevalence of the Iberian Poleward Current and sometimes the Azores Current, although the subtropical waters were more oligotrophic during the MIS 2 deglaciation than the MIS 12 one. Synchronizing our records according to the precession cycles aligns the early-to-mid Holocene with the second, warmer phase of MIS 11c. During both these intervals, the western Iberian Margin was mainly affected by the Iberian Poleward Current that transported more temperate-warm, mesotrophic surface waters during MIS 11c than during the early-to-mid Holocene. During the early to mid-Holocene the Iberian Margin endured incursions of colder surface waters that did not occur during MIS 11c allowing us to hypothesize that the studied site experienced, from a paleoceanographic point of view, a more stable period during MIS 11c than the early Holocene. Finally, spectral analysis suggests the role of full, half and fourth precession components in driving surface-ocean variability during MIS 11 and during the last 24 kyr BP.
- Gorongosa by the sea: First Miocene fossil sites from the Urema Rift, central Mozambique, and their coastal paleoenvironmental and paleoecological contextsPublication . Habermann, Jörg M.; Alberti, Matthias; Aldeias, Vera; Alemseged, Zeresenay; Archer, Will; Bamford, Marion; Biro, Dora; Braun, David R.; Capelli, Cristian; Cunha, Eugenia; da Silva, Maria Ferreira; Luedecke, Tina; Madiquida, Hilario; Martinez, Felipe I.; Mathe, Jacinto; Negash, Enquye; Paulo, Luis M.; Pinto, Maria; Stalmans, Marc; Regala, Frederico Tata; Wynn, Jonathan G.; Bobe, Rene; Carvalho, SusanaThe East African Rift System (EARS) has played a central role in our understanding of human origins and vertebrate evolution in the late Cenozoic of Africa. However, the distribution of fossil sites along the rift is highly biased towards its northern extent, and the types of paleoenvironments are primarily restricted to fluvial and lacustrine settings. Here we report the discovery of the first fossil sites from the Urema Rift at Gorongosa National Park (central Mozambique) at the southern end of the EARS, and reconstruct environmental contexts of the fossils. In situ and surface fossils from the lower member of the Mazamba Formation, estimated to be of Miocene age, comprise mammals, reptiles, fishes, invertebrates, palms, and dicot trees. Fossil and geological evidence indicates a coastal-plain paleoenvironmental mosaic of riverine forest/woodland and estuarine habitats that represent the first coastal biomes identified in the Neogene EARS context. Receiving continental sediment from source terranes west of today's Urema Graben, estuarine sequences accumulated prior to rifting as compound incised-valley fills on a low-gradient coastal plain following transgression. Modern environmental analogues are extremely productive habitats for marine and terrestrial fauna, including primates. Thus, our discoveries raise the possibility that the Miocene coastal landscapes of Gorongosa were ecologically-favorable habitats for primates, providing relatively stable maritime climate and ecosystem conditions, year-round freshwater availability, and food both from terrestrial and marine sources. The emerging fossil record from Gorongosa is beginning to fill an important gap in the paleobiogeography of Africa as no fossil sites of Neogene age have previously been reported from the southernmost part of the EARS. Furthermore, this unique window into past continental-margin ecosystems of central Mozambique may allow us to test key paleobiogeographic hypotheses during critical periods of primate evolution.
- Skills for disruptive digital businessPublication . Sousa, Maria José; Rocha, AlvaroThis article analyses the concept of skills and also investigates the skills needed to create and manage disruptive digital business which is emerging from the IT evolution. The primary purpose is to identify skills which need to be developed to manage a disruptive digital business. The technologies, disruptive business and the skills needed by managers were identified through content analysis of semi-structured interviews with seven IT specialists. To analyze the development of the level of skills needed was conducted an online survey with managers and the final output of the research is a proposal of a model of skills' development for managers of the disruptive business. Ultimately, the result of this research are the lessons uncovered and the proposal made for a model of skills development for disruptive business managers, which identifies three types/categories of skills needing development - innovation, leadership, and management.
- Increased evolutionary rates and conserved transcriptional response following allopolyploidization in brown algaePublication . Sousa, Filipe; Neiva, J.; Martins, Neusa; Jacinto, Rita; Anderson, Laura; Raimondi, Peter T.; Serrao, Ester; Pearson, GarethGenome mergers between independently evolving lineages, via allopolyploidy, can potentially lead to instantaneous sympatric speciation. However, little is known about the consequences of allopolyploidy and the resultant "genome shock" on genome evolution and expression beyond the plant and fungal branches of the Tree of Life. The aim of this study was to compare substitution rates and gene expression patterns in two allopolyploid brown algae (Phaeophyceae and Heterokonta) and their progenitors in the genus Pelvetiopsis N. L. Gardner in the north-east Pacific, and to date their relationships. We used RNA-seq data, all potential orthologues, and putative single-copy loci for phylogenomic, divergence, and gene expression analyses. The multispecies coalescent placed the origin of allopolyploids in the late Pleistocene (0.35-0.05 Ma). Homoeologues displayed increased nonsynonymous divergence compared with parental orthologues, consistent with relaxed selective constraint following allopolyploidization, including for genes with no evidence of pseudogenization or neofunctionalization. Patterns of homoeologue-orthologue expression conservation and expression level dominance were largely shared with both natural plant and fungal allopolyploids. Our results provide further support for common cross-Kingdom patterns of allopolyploid genome evolution and transcriptional responses, here in the evolutionarily distinct marine heterokont brown algae.
- Investigation of luxury values in shopping tourism using a fuzzy-set approachPublication . Correia, Antónia; Kozak, Metin; Kim, Seongseop (Sam)This exploratory study, based on 316 questionnaires completed by mainland Chinese tourists in Hong Kong, investigates whether certain conditions are sufficient to motivate tourists' decisions to buy luxury products. The results of fuzzy-set qualitative comparative analysis reveal that a combination of four conditions (emotional attachment, fashion leadership, prestige sensitivity, and social value) is sufficient to influence tourists to shop for luxury brands. It must be pointed out, however, that the four conditions are unnecessary in some social and shopping contexts, and thus the findings do not reject the possibility that alternative conditions may also influence luxury shopping decisions. This study using fuzzy-set theory demonstrates that tourists who score highly in all four conditions constitute an almost perfect subcategory of luxury shoppers. Therefore, it is recommended that other scholars examine the conditions that are sufficient to influence tourists rather than concentrating on other factors.
- The fishes of St Helena Island, central Atlantic Ocean-new records and an annotated check-listPublication . Brown, Judith; Beard, Annalea; Clingham, Elizabeth; Fricke, Ronald; Henry, Leeann; Wirtz, PeterA check-list of the fishes of St Helena Island is presented. The following species are recorded for the first time from St. Helena Island: Rhincodon typus, Mobula tarapacana, Muraena melanotis, Caranx latus, Seriola rivoliana, Balistes capriscus, Lutjanus jocu, Centropyge aurantonotus, Acanthurus coeruleus, Lepidocybium flavobrunneum, Tetrapturus pfluegeri, Coelorinchus geronimo, Pentaceros richardsoni, Gephyroberyx darwinii, Brotula cf multibarbata, Poromitra crassiceps, Echiostoma barbatum, Malacosteus niger, Pachystomias microdon. Including these nineteen new records there are 189 fish species currently known from St Helena. Three of them appear to be undescribed. Eight species and two subspecies are currently considered endemic to St. Helena Island.