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- Effects of planting density and soil management on pitaya (S. undatus) yield in an outdoor plantationPublication . Trindade, Ana Rita; Matias, Pedro; Zarcos Duarte, Beatriz; Trindade, D.; Duarte, AmilcarIn regions with advanced pitaya (Selenicereus spp.) cultivation, the crop is popular due to its rapid production cycle and favourable fruit prices. The Mediterranean offers favourable cultivation conditions, but more research is needed on agronomic practices like spacing and soil management, which affect profitability and sustainability. A trial was conducted in an outdoor plantation to assess the effect of two planting spacings (PS-0.5, plant spacing of 0.5 m; PS-1, plant spacing of 1 m) and two weed management methods (SC-BS, soil cover with black screen; SC-M, soil cover with mulch) on pitaya production parameters (fruits plant‑1; kg plant‑1; average fruit weight; kg m‑2), in the first and second production years. The use of mulching (SC-M) resulted in a higher number of fruits plant‑1 compared to the black screen (SC-BS), but this effect was only observed in the 1st production year. However, PS-1 and SC-BS obtained higher yield (kg plant‑1) compared to the PS-0.5 and SC-M plants. Neither plant spacing nor soil cover strongly influenced fruit size variation over time. Ultimately, the spacing that proved to be the most productive was the tightest (PS-0.5) with an average value of 0.82±0.04 kg m‑2, compared to PS-1 (0.60±0.04 kg m‑2). Results show that pitaya productivity is increasing annually, even under tighter spacings. Adding organic matter to the soil significantly boosts production, and consistent mulching improves soil fertility and controls weeds efficiently.
- Efficacy of slow sand filtration enriched with trichoderma atroviride in the control of fusarium oxysporum in soilless cultivation systemsPublication . Matias, Pedro; Guerreiro David Coelho, Luísa Isabel; Reis, MárioOn a planet intending to move toward carbon neutrality while ensuring food security, maximizing water and nutrient use efficiency in agriculture is essential. Soilless cultivation offers a promising solution for food production, yet in substrate-based systems, excess nutrient solution (drainage) is often discarded to maintain phytosanitary safety, resulting in considerable water and nutrient waste. Reusing this drainage requires disinfection to eliminate pathogens. Among available methods, slow sand filtration (SSF) is ecological, economical, and simple, showing strong biological control potential, though not always fully effective against Fusarium oxysporum. Trichoderma atroviride, an antagonistic fungus, may enhance SSF performance. Its antagonistic capacity was evaluated in vitro via direct confrontation assays and in vivo using a closed-loop soilless cucumber cultivation system with eight treatment combinations of SSF, T. atroviride, and F. oxysporum. SSF reduced F. oxysporum incidence by approximately 48%, T. atroviride in irrigation by 44%, and SSF enriched with T. atroviride reached 58% disease incidence reduction, though this increase was not statistically significant. These results confirm that both SSF and T. atroviride can partially suppress F. oxysporum, but further optimization is needed for consistent and complete pathogen control.
- From Lignocellulosic residues to protein sources: insights into biomass pre-treatments and conversionPublication . Vera dos Anjos, Isabela; Coelho, Natacha; Duarte, Hugo; Proença, Diogo Neves; Duarte, Maria F.; Jorge de Barros, Raúl José; Raposo, Sara; Gonçalves, Sandra; Romano, Anabela; Medronho, BrunoWith the global population steadily rising, the demand for sustainable protein sources has become increasingly urgent. Traditional animal- and plant-based proteins face challenges related to scalability, resource efficiency, and environmental impact. In this context, single-cell protein has emerged as a promising alternative. Derived from microorganisms such as algae, bacteria, fungi, and yeast, single-cell protein offers a high nutritional profileincluding all essential amino acids and vitamins—while enabling rapid production, minimal land and water requirements, and no generation of greenhouse gas emissions. A particularly compelling advantage of single-cell protein is its ability to be produced from agro-industrial waste, converting low-cost residues into valuable nutritional resources and contributing to environmental sustainability. Among these waste streams, lignocellulosic biomass from agricultural and forestry residues stands out as a renewable, biodegradable, and abundant feedstock. This review explores the potential of lignocellulosic waste as a substrate for single-cell protein production, emphasizing both its environmental advantages and nutritional value. It highlights the single-cell protein role as a sustainable and scalable alternative to conventional protein sources. The review also identifies key scientific, economic, and regulatory challenges, and recognizes the importance of targeted investments, particularly in policy development, public awareness, and technological innovation, to enable the broader adoption and acceptance of single-cell protein -based products.
- Preliminary insight into winter native fish assemblages in guadiana estuary salt marshes coping with environmental variability and non-indigenous fish introductionPublication . Goncalves, Renata; Teodosio, Maria; Cruz, Joana; Ben-Hamadou, Radhouan; Correia, Ana Dulce; Chicharo, LuisThis work aims to undertake a preliminary characterization of winter fish assemblages in the salt marsh areas of Guadiana lower estuary (South-East Portugal) and discusses the potential risks of habitat dominance by a non-indigenous species (NIS). To this effect, six field campaigns were carried out in four sampling sites during winter season targeting the collection of fish species. A total of 48 samples were collected. Individuals from seven different taxa (marine and estuarine) were collected, although the assemblage was dominated by two estuarine species—the native Pomatoschistus sp. (goby) and the NIS Fundulus heteroclitus (mummichog). Goby was the most abundant taxa in the majority of salt marsh habitats, except for one specific, marsh pool, where extreme environmental conditions were registered, namely high temperature and salinity. Such conditions may have boosted the intrusion of mummichog in this area. This species is well adapted to a wide range of abiotic factors enabling them to colonize habitats where no predators inhabit. Impacts of mummichog introduction in the Guadiana salt marsh area are still unpredictable since this is the first time they have been recorded in such high density. Nevertheless, in scenarios of increased anthropogenic pressure and, consequently, habitat degradation, there is a potential risk of mummichog spreading to other habitats and therefore competing for space and food resources with native species.