DE NOVO FORMATION OF AN EPITHELIUM DURING DROSOPHILA BLASTODERM CELLULARIZATION: A GENETIC AND BIOCHEMICAL APPROACH

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Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells

Publication . Guilgur, Leonardo G.; Prudencio, Pedro; Ferreira, Tania; Pimenta-Marques, Ana Rita; Goncalo Martinho, Rui

Epithelial cells mostly orient the spindle along the plane of the epithelium (planar orientation) for mitosis to produce two identical daughter cells. The correct orientation of the spindle relies on the interaction between cortical polarity components and astral microtubules. Recent studies in mammalian tissue culture cells suggest that the apically localised atypical protein kinase C (aPKC) is important for the planar orientation of the mitotic spindle in dividing epithelial cells. Yet, in chicken neuroepithelial cells, aPKC is not required in vivo for spindle orientation, and it has been proposed that the polarization cues vary between different epithelial cell types and/or developmental processes. In order to investigate whether Drosophila aPKC is required for spindle orientation during symmetric division of epithelial cells, we took advantage of a previously isolated temperature-sensitive allele of aPKC. We showed that Drosophila aPKC is required in vivo for spindle planar orientation and apical exclusion of Pins (Raps). This suggests that the cortical cues necessary for spindle orientation are not only conserved between Drosophila and mammalian cells, but are also similar to those required for spindle apicobasal orientation during asymmetric cell division.

2012-02Artigo científico

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Fundação para a Ciência e a Tecnologia

Número da atribuição

SFRH/BD/28767/2006