Untitled

Funder

Organizational Unit

Publications

Fatty acid and lipid class metabolism of common octopus (Octopus vulgaris) and of European cuttlefish (Sepia officinalis) at early life stages

Publication . Reis, Diana Filipa Botelho; Andrade, José Pedro; Sykes, António V.; Rodriguez, Covadonga

Octopus vulgaris and Sepia officinalis are two species of cephalopods that have been recognised with a great potential for aquaculture.

2016-07-18Doctoral thesis

Open access

Comparative study on fatty acid metabolism of early stages of two crustacean species: Artemia sp. metanauplii and Grapsus adscensionis zoeae, as live prey for marine animals

Publication . Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Navarro, Juan C.; Tocher, Douglas R.; Andrade, José Pedro; Sykes, António V.; Rodríguez, Covadonga

The present study compared the lipid composition and in vivo capability of Artemia sp. metanauplii (the main live prey used in aquaculture) and Grapsus adscensionis zoeae (as a wild zooplankton model) to metabolise unsaturated fatty acids. The two species were incubated in vivo with 0.3μM of individual [1-14C]fatty acids (FA) including 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) and 22:6n-3 (DHA) bound to bovine serum albumin (BSA). Compared to metanauplii, zoeae contained twice the content of polar lipids (PL) and eight-fold the content of long-chain polyunsaturated fatty acids (LC-PUFA). Artemia sp. metanauplii showed increased short chain fatty acid de novo synthesis from beta-oxidation of [1-14C]LC-PUFA, preferentially DHA. Of the LC-PUFA, DHA showed the highest esterification rate into Artemia sp. triacylglycerols. In contrast, in Grapsus zoeae [1-14C]DHA displayed the highest transformation rate into longer chain-length FAs and was preferentially esterified into PL. EPA and ARA, tended to be more easily incorporated and/or retained than DHA in Artemia sp. Moreover, both EPA and ARA were preferentially esterified into Artemia PL, which theoretically would favour their bioavailability to the larvae. In addition to the inherent better nutritional value of Grapsus zoeae due to their intrinsic lipid composition, the changes taking place after the lipid incorporation, point at two distinct models of lipid metabolism that indicate zoeae as a more suitable prey than Artemia sp. for the feeding of marine animals.

2017Journal article

Restricted access

Effect of of Artemia inherent fatty acid metabolism on the bioavailability of essential fatty acids for Octopus vulgaris paralarvae development

Publication . B Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Garrido, Diego; Andrade, José Pedro; Sykes, Antonio V.; Rodriguez, Covadonga

The aim of the present study was to determine the effect of Artemia metanauplii endogenous fatty acid (FA) metabolism in the actual availability of dietary essential fatty acids (EFA) for Octopus vulgaris paralarvae development. To this end, both Artemia metanauplii inherent radiolabelled FA metabolism and the Octopus vulgaris paralarvae FA metabolism, after being fed with Artemia metanauplii incubated with radiolabelled. FAs, were determined. Metanauplii were incubated in tissue culture plates during 12 h, with 0.3 mu M of a [1-C-14]FA, including either 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA), added individually to separate wells as their potassium salts bound to bovine serum albumin. A total of 3600 one-day-old paralarvae were reared up to 24 h in order to ensure the ingestion of a minimum amount of the labelled Artemia. Paralarvae rearing was performed in 4 L cylinder conical tanks at a density of 25 paralarvae L-1. Labelled Artemia metanauplii were added to each paralarvae rearing tank according to a specific [1-C-14]FA treatment. Artemia in vivo metabolism results showed a preferential catabolism of DHA, which was translated into (1) the lower incorporation of this FA into Artemia total lipids (TL)

2019-02Journal article

Restricted access

Preliminary results on light conditions manipulation in Octopus vulgaris (Cuvier, 1797) paralarval rearing

Publication . Garrido, Diego; Reis, Diana; Orol, Diego; Gonçalves, Rui A.; Martín, M.; Sykes, António V.; Rodríguez, Covadonga; Felipe, Beatriz; Zheng, Xiaodong; Lagos, Luis; Almansa, Eduardo

High paralarvae mortality is a major bottleneck currently hindering the control over the lifecycle of common octopus (Octopus vulgaris Cuvier, 1797). It is believed that this problem might be related to either zoo-technical and/or nutritional aspects. The present paper is focused on the study of different zoo-technical aspects related to light conditions on the rearing of paralarvae, including the effects of polarization in prey ingestion, the use of a blue filter to simulate natural conditions, and the use of focused light to avoid reflections of the rearing tank’s walls. In the first experiment, O. vulgaris paralarvae ingestion of Artemia sp. and copepods (Tisbe sp.) was assessed under either normal or polarized light. In the second experiment, the effect of a blue filter with natural light or focused artificial light on growth and mortality was assessed over 15 days of rearing. Ingestion rate was not influenced by light polarization. Nonetheless, a significantly higher ingestion of Artemia sp. with respect to copepods was observed. The blue filter promoted the use of natural light conditions in Octopus paralarval culture, while focused light reduced the collision of the paralarvae against the walls. However, no significant differences were found in paralarval growth nor survival.

2017Journal article

Open access

In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes

Publication . Reis, Diana; Acosta, N. G.; Almansa, E.; Navarro, J. C.; Tocher, D. R.; Monroig, O.; Andrade, José Pedro; Sykes, António V.; Rodríguez, C.

The highmortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36‰). Incubations were performed with gentle stirring at 21 °C for 6 h with 0.2 μCi (0.3 μM) of [1−14C]-labelled FA including 18:1n−9, 18:2n−6, 18:3n−3, 20:4n−6 (ARA), 20:5n−3 (EPA) or 22:6n−3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum albumin (BSA). A control treatmentwithout [1−14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1−14C]DHA and [1−14C]C18 FA substratesweremainly esterified into phosphatidylcholine, while [1−14C]ARA and [1−14C]EPA were esterified into phosphatidylethanolamine.

2014Journal article

Restricted access