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Projeto de investigação
Improving the flow in the pipeline of the next generation of marine biodiscovery scientists
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Genomics-metabolomics profiling disclosed marine Vvbrio spartinae 3.6 as a producer of a new branched side chain prodigiosin
Publication . Vitale, Giovanni Andrea; Sciarretta, Martina; Esposito, Fortunato Palma; January, Grant Garren; Giaccio, Marianna; Bunk, Boyke; Sproeer, Cathrin; Bajerski, Felizitas; Power, Deborah; Festa, Carmen; Monti, Maria Chiara; D'Auria, Maria Valeria; de Pascale, Donatella
A wide range of prescreening tests for antimicrobial activity of 59 bacterial isolates from sediments of Ria Formosa Lagoon (Algarve, Portugal) disclosed Vibrio spartinae 3.6 as the most active antibacterial producing strain. This bacterial strain, which has not previously been submitted for chemical profiling, was subjected to de novo whole genome sequencing, which aided in the discovery and elucidation of a prodigiosin biosynthetic gene cluster that was predicted by the bioinformatic tool KEGG BlastKoala. Comparative genomics led to the identification of a new membrane di-iron oxygenase-like enzyme, annotated as Vspart_02107, which is likely to be involved in the biosynthesis of cycloprodigiosin and analogues. The combined genomicsmetabolomics profiling of the strain led to the isolation and identification of one new branched-chain prodigiosin (5) and to the detection of two new cyclic forms. Furthermore, the evaluation of the minimum inhibitory concentrations disclosed the major prodigiosin as very effective against multi-drug-resistant pathogens including Stenotrophomonas maltophilia, a clinical isolate of Listeria monocytogenes, as well as some human pathogens reported by the World Health Organization as prioritized targets.
Reproductive dynamics of the sympatric topshells Phorcus lineatus and Phorcus sauciatus (Gastropoda: Trochidae) on a rocky intertidal coast in southern Portugal
Publication . Moura, Paula; Vasconcelos, Paulo; Pereira, Fábio; Carvalho, André N.; Gaspar, Miguel
The present study aimed to describe the reproductive cycle of the topshells Phorcus lineatus and Phorcus sauciatus from an intertidal rocky shore in the Algarve, southern Portugal. Based on two years of monthly sampling performed between January 2017 and December 2018, the study involved analyses of gonad histology and estimation of the mean gonadal index, aiming to investigate their relationship with environmental variables. Both topshell species are dioecious and showed synchrony in the reproductive dynamics between sexes. Indeed, the reproductive cycle of P. lineatus and P. sauciatus exhibited similar patterns, with scarcity of inactive gonads and prolonged periods with mature and spawning individuals. The gonadal index closely reflected the species’ reproductive cycle, which is apparently influenced by seasonal fluctuations in air and seawater temperatures. Additionally, some atmospheric and oceanographic conditions, such as daylight duration and wave height, were also correlated with gonad ripening and spawning of P. lineatus, and wind speed with gonad ripening and spawning of P. sauciatus. Overall, the present study provided valuable information to propose harvesting management measures aiming to ensure the sustainable exploitation of this shellfish resource, namely a closed season in the harvesting of both topshell species in September–October, covering their spawning peak in southern Portugal.
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Entidade financiadora
European Commission
Programa de financiamento
H2020
Número da atribuição
721421