Measuring dielectric constants of lipid bilayers: monitoring scatter effects in pyrene fluorescence

Cristo, Joana; Silva, A.; Martins, J.

http://hdl.handle.net/10400.1/13068

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Authors

Cristo, Joana

Silva, A.

Martins, J.

Abstract(s)

Pyrene (Py) fluorescence is expedient to study biophysical phenomena in lipid bilayer due to high quantum yield, long lifetime and precise solvatochromic effects [1]. The physicalchemical properties of Py settle its location in the ordered section of the methylenic palisade stating the values of dielectric constants of bilayers, averaged transversally in space (the longest axis of Py, 9.2 ˚A) and laterally in time (due to lateral diffusion of Py during its lifetime, 150 ns) [2].