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Measuring dielectric constants of lipid bilayers: monitoring scatter effects in pyrene fluorescence
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Resumo(s)
Pyrene (Py) fluorescence is expedient to study biophysical
phenomena in lipid bilayer due to high quantum yield, long
lifetime and precise solvatochromic effects [1]. The physicalchemical properties of Py settle its location in the ordered
section of the methylenic palisade stating the values of dielectric constants of bilayers, averaged transversally in space (the
longest axis of Py, 9.2 ˚A) and laterally in time (due to lateral
diffusion of Py during its lifetime, 150 ns) [2].