Publication
Incorporation of capric acid in pumpkin seed oil by sn-1,3 regioselective lipase-catalyzed acidolysis
| dc.contributor.author | Sousa, Vanessa | |
| dc.contributor.author | Campos, Vitor | |
| dc.contributor.author | Nunes, Patricia | |
| dc.contributor.author | Pires-Cabral, Paula | |
| dc.date.accessioned | 2018-12-07T14:57:53Z | |
| dc.date.available | 2018-12-07T14:57:53Z | |
| dc.date.issued | 2018-06 | |
| dc.description.abstract | Structured lipids (SLs) are novel triacylglycerols obtained by changing the native fatty acid (FA) profiles or by the incorporation of a new desired FA in the acylglycerol backbone. These modified fats present important medical and functional properties for food applications. This work aimed to synthetize a MLM-type SL, which consists of triacylglycerols containing a medium-chain FA (M) at sn-1,3 positions and a long-chain FA (L) at sn-2 position, by acidolysis of pumpkin seed oil with capric acid, catalyzed by a commercial lipase preparation from Thermomyces lanuginosa (Lipozyme TL IM). Reactions were performed at 45 degrees C, in solvent-free media, at 1: 2 molar ratio (pumpkin seed oil: capric acid) and a fixed amount of immobilized lipase of 5%, 10%, 15% or 20%. Incorporations of C10: 0 increased with time up to 31 h (29.9 +/- 0.7 mol-%) when 5% lipase load was used. Significant differences were only observed between the results obtained with 5 and 20% of biocatalyst load. The subsequent experiment was carried out with 5% lipase load, at 45 degrees C, 1: 2 molar ratio and in the presence of n-hexane. The results showed slightly higher incorporation yields in the presence of solvent, namely at 48 h-reaction (34.7 +/- 1.0 mol-%). However, since the structured lipids are to be used in food products, together with environmental and economic concerns, solvent-free systems are preferred. In this study, the synthesis of a MLM-type SL from pumpkin seed oil for food uses was well succeeded. | |
| dc.identifier.doi | 10.1051/ocl/2018004 | |
| dc.identifier.issn | 1258-8210 | |
| dc.identifier.issn | 2257-6614 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/11750 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Edp Sciences S A | |
| dc.subject | Cucurbita-Pepo L | |
| dc.subject | Structured Lipids | |
| dc.subject | Chemical-Composition | |
| dc.subject | Fatty-Acids | |
| dc.subject | Interesterification | |
| dc.subject | Triacylglycerols | |
| dc.title | Incorporation of capric acid in pumpkin seed oil by sn-1,3 regioselective lipase-catalyzed acidolysis | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 3 | |
| oaire.citation.startPage | A302 | |
| oaire.citation.title | Ocl-Oilseeds and Fats Crops and Lipids | |
| oaire.citation.volume | 25 | |
| person.familyName | Pires-Cabral | |
| person.givenName | Paula | |
| person.identifier.orcid | 0000-0001-9567-2165 | |
| person.identifier.scopus-author-id | 8675090400 | |
| rcaap.rights | openAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | a6dad315-0960-4e2a-8570-25c357da5456 | |
| relation.isAuthorOfPublication.latestForDiscovery | a6dad315-0960-4e2a-8570-25c357da5456 |
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