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Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2

2012-10Journal article Open access
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dc.contributor.authorYoshiba, Satoko
dc.contributor.authorShiratori, Hidetaka
dc.contributor.authorKuo, Ivana Y.
dc.contributor.authorKawasumi, Aiko
dc.contributor.authorShinohara, Kyosuke
dc.contributor.authorNonaka, Shigenori
dc.contributor.authorAsai, Yasuko
dc.contributor.authorSasaki, Genta
dc.contributor.authorBelo, José A.
dc.contributor.authorSasaki, Hiroshi
dc.contributor.authorNakai, Junichi
dc.contributor.authorDworniczak, Bernd
dc.contributor.authorEhrlich, Barbara E.
dc.contributor.authorPennekamp, Petra
dc.contributor.authorHamada, Hiroshi
dc.date.accessioned2018-12-07T14:58:07Z
dc.date.available2018-12-07T14:58:07Z
dc.date.issued2012-10
dc.description.abstractUnidirectional fluid flow plays an essential role in the breaking of left-right (L-R) symmetry in mouse embryos, but it has remained unclear how the flow is sensed by the embryo. We report that the Ca2+ channel Polycystin-2 (Pkd2) is required specifically in the perinodal crown cells for sensing the nodal flow. Examination of mutant forms of Pkd2 shows that the ciliary localization of Pkd2 is essential for correct L-R patterning. Whereas Kif3a mutant embryos, which lack all cilia, failed to respond to an artificial flow, restoration of primary cilia in crown cells rescued the response to the flow. Our results thus suggest that nodal flow is sensed in a manner dependent on Pkd2 by the cilia of crown cells located at the edge of the node.
dc.description.sponsorshipCREST of the Japan Science and Technology Corporation; NIH [P30 DK090744]; Human Frontier Science Program [ST00246/2003C]; Deutsche Forschungsgemeinschaft [PE 853/2]; Japan Society for the Promotion of Science; American Heart Association [R10682]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doihttps://doi.org/10.1126/science.1222538
dc.identifier.issn0036-8075
dc.identifier.urihttp://hdl.handle.net/10400.1/11866
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Association for the Advancement of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPolycystic kidney disease
dc.subjectLeft-right asymmetry
dc.subjectLeft-right axis
dc.subjectMice
dc.subjectChannel
dc.subjectProtein
dc.subjectExpression
dc.subjectRelease
dc.subjectLacking
dc.subjectKif3A
dc.titleCilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage231
oaire.citation.issue6104
oaire.citation.startPage226
oaire.citation.titleScience
oaire.citation.volume338
person.familyNameBelo
person.givenNameJosé A.
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6602141392
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscovery4bebeb48-6fac-41a9-8363-f849def5e019

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