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Ectopic expression of CITED2 prior to reprogramming, promotes and homogenises the conversion of somatic cells into induced pluripotent stem cells

2017-09Journal article Restricted
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dc.contributor.authorCharneca, João
dc.contributor.authorMatias, Ana Catarina
dc.contributor.authorEscapa, Ana Luisa
dc.contributor.authorFernandes, Catarina
dc.contributor.authorAlves, Andre
dc.contributor.authorSantos, João
dc.contributor.authorNascimento, Rita
dc.contributor.authorBragança, José
dc.date.accessioned2019-11-20T15:07:21Z
dc.date.available2019-11-20T15:07:21Z
dc.date.issued2017-09
dc.description.abstractCited2 plays crucial roles in mouse embryonic stem cells self-renewal, the initiation of the somatic reprogramming process into induced pluripotent stem cells (iPSC) and the suppression of cell senescence. Here, we investigated the potential of CITED2 expression in combination with the Oct4, Sox2, Klf4 and c-Myc factors for reprogramming of primary mouse embryonic fibroblasts (MEF) at passage 2 and 4. The ectopic CITED2 expression in primary MEF prior to the onset of the reprogramming process, generated iPSC with less variability in the expression of endogenous pluripotency-related genes. In contrast, part of the MEF reprogrammed without ectopic expression of CITED2 at passage 4 originated partially reprogrammed iPSC or pre-iPSC. However, the overexpression of CITED2 in the pre-iPSC was insufficient to complete the reprogramming process into iPSC. These results indicated that ectopic CITED2 expression at the onset of the reprogramming process in combination with the reprogramming factors promotes a complete and homogeneous conversion of somatic cells into iPSC.
dc.description.sponsorshipFCT - Fundacao para a Ciencia e a Tecnologia [PTDC/BIM-MEC/4762/2014]
dc.description.sponsorshipMerck Sharp & Dhome Foundation - Portugal
dc.identifier.doi10.1016/j.yexcr.2017.07.002
dc.identifier.issn0014-4827
dc.identifier.issn1090-2422
dc.identifier.urihttp://hdl.handle.net/10400.1/12993
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Inc
dc.subjectGround-state pluripotency
dc.subjectSelf-renewal
dc.subjectBinding-Protein
dc.subjectMouse
dc.subjectGeneration
dc.subjectCoactivator
dc.subjectFibroblasts
dc.subjectHif-1-Alpha
dc.subjectDifferentiation
dc.subjectProliferation
dc.titleEctopic expression of CITED2 prior to reprogramming, promotes and homogenises the conversion of somatic cells into induced pluripotent stem cells
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-ENB%2F111702%2F2009/PT
oaire.citation.endPage300
oaire.citation.issue2
oaire.citation.startPage290
oaire.citation.titleExperimental Cell Research
oaire.citation.volume358
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameCharneca
person.familyNameMatias
person.familyNameFernandes
person.familyNameSantos
person.familyNameBragança
person.givenNameJoão
person.givenNameAna Catarina
person.givenNameCatarina
person.givenNameJoão
person.givenNameJosé
person.identifier2373709
person.identifier.ciencia-id9F1D-9356-60AE
person.identifier.ciencia-id701D-019D-0779
person.identifier.ciencia-id6411-7CD1-697E
person.identifier.ciencia-idB713-B787-0A9E
person.identifier.ciencia-idAC1D-FA9D-F66F
person.identifier.orcid0000-0002-3154-5711
person.identifier.orcid0000-0002-6385-9117
person.identifier.orcid0000-0002-6649-4889
person.identifier.orcid0000-0002-1218-368X
person.identifier.orcid0000-0001-9566-400X
person.identifier.ridA-9430-2014
person.identifier.scopus-author-id55232110200
person.identifier.scopus-author-id6602220001
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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