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Combining hyaluronic acid with chitosan enhances gene delivery

2014Journal article Open access
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dc.contributor.authorOliveira, Ana
dc.contributor.authorBitoque, Diogo
dc.contributor.authorSilva, Gabriela
dc.date.accessioned2018-12-07T14:58:27Z
dc.date.available2018-12-07T14:58:27Z
dc.date.issued2014
dc.description.abstractThe low gene transfer efficiency of chitosan-DNA polyplexes is a consequence of their high stability and consequent slow DNA release. The incorporation of an anionic polymer is believed to loosen chitosan interactions with DNA and thus promote higher transfection efficiencies. In this work, several formulations of chitosan-DNA polyplexes incorporating hyaluronic acid were prepared and characterized for their gene transfection efficiency on both HEK293 and retinal pigment epithelial cells. The different polyplex formulations showed morphology, size, and charge compatible with a role in gene delivery. The incorporation of hyaluronic acid rendered the formulations less stable, as was the goal, but it did not affect the loading and protection of the DNA. Compared with chitosan alone, the transfection efficiency had a 4-fold improvement, which was attributed to the presence of hyaluronic acid. Overall, our hybrid chitosan-hyaluronic acid polyplexes showed a significant improvement of the efficiency of chitosan-based nonviral vectors in vitro, suggesting that this strategy can further improve the transfection efficiency of nonviral vectors.
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia [SFRH/BD/52424/2013]; Marie Curie Reintegration Grant [PIRG-GA-2009-249314]
dc.identifier.doi10.1155/2014/246347
dc.identifier.issn1687-4110
dc.identifier.issn1687-4129
dc.identifier.urihttp://hdl.handle.net/10400.1/12033
dc.language.isoeng
dc.peerreviewedyes
dc.publisherHindawi Publishing Corporation
dc.relationDEVELOPMENT OF NON-VIRAL VECTORS FOR GENE THERAPY FOR PATHOLOGIES OF THE RETINA
dc.subjectTransfection efficiency
dc.subjectIn-vivo
dc.subjectNanoparticles
dc.subjectExpression
dc.subjectCarriers
dc.subjectTherapy
dc.subjectCells
dc.subjectCd44
dc.titleCombining hyaluronic acid with chitosan enhances gene delivery
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF NON-VIRAL VECTORS FOR GENE THERAPY FOR PATHOLOGIES OF THE RETINA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BEB%2F098475%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70318%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.startPage246347
oaire.citation.titleJournal of Nanomaterials
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameOliveira
person.familyNameBitoque
person.familyNameSilva
person.givenNameAna
person.givenNameDiogo
person.givenNameGabriela
person.identifier2140108
person.identifier.ciencia-idCA17-42CB-F11B
person.identifier.ciencia-idD81C-6457-7F28
person.identifier.ciencia-idDC1E-F164-FDD9
person.identifier.orcid0000-0003-3162-250X
person.identifier.orcid0000-0002-6536-6578
person.identifier.orcid0000-0001-5946-1918
person.identifier.ridF-4409-2012
person.identifier.scopus-author-id55989431200
person.identifier.scopus-author-id55755464000
person.identifier.scopus-author-id7101647767
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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