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Drosophila protein kinase N (Pkn) is a negative regulator of actin-myosin activity during oogenesis

2014-10Journal article Open access
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dc.contributor.authorFerreira, Tania
dc.contributor.authorPrudencio, Pedro
dc.contributor.authorMartinho, Rui Goncalo
dc.date.accessioned2018-12-07T14:58:07Z
dc.date.available2018-12-07T14:58:07Z
dc.date.issued2014-10
dc.description.abstractNurse cell dumping is an actin-myosin based process, where 15 nurse cells of a given egg chamber contract and transfer their cytoplasmic content through the ring canals into the growing oocyte. We isolated two mutant alleles of protein kinase N (pkn) and showed that Pkn negatively-regulates activation of the actin-myosin cytoskeleton during the onset of dumping. Using live-cell imaging analysis we observed that nurse cell dumping rates sharply increase during the onset of fast dumping. Such rate increase was severely impaired in pkn mutant nurse cells due to excessive nurse cell actin-myosin activity and/or loss of tissue integrity. Our work demonstrates that the transition between slow and fast dumping is a discrete event, with at least a five to six-fold dumping rate increase. We show that Pkn negatively regulates nurse cell actin-myosin activity. This is likely to be important for directional cytoplasmic flow. We propose Pim provides a negative feedback loop to help avoid excessive contractility after local activation of Rho GTPase. (C) 2014 Elsevier Inc. All rights reserved.
dc.description.sponsorshipNational Portuguese Funding through Grants FCT-Fundacao para Ciencia e Tecnologia [PTDC/SAU-BID/111796/2009, PTDC/BIA-BCM/111822/2009, PTDC/BBB-BQB/0712/2012, PEst-OE/EQB/LA0023/2013]; Fundacao para Ciencia e Tecnologia [SFRH/BD/37587/2007]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.ydbio.2014.08.008
dc.identifier.issn0012-1606
dc.identifier.issn1095-564X
dc.identifier.urihttp://hdl.handle.net/10400.1/11870
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Elsevier Science
dc.relationDEFINING NOVEL MECHANISMS REQUIRED FOR THE FORMATION AND MORPHOGENESIS OF A POLARIZED EPITHELIUM
dc.relationCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCapping protein
dc.subjectDorsal closure
dc.subjectPotential effector
dc.subjectCatalytic domain
dc.subjectEpithelial-cells
dc.subjectStress fibers
dc.subjectRho
dc.subjectCytoskeleton
dc.subjectPhosphatase
dc.subjectMelanogaster
dc.titleDrosophila protein kinase N (Pkn) is a negative regulator of actin-myosin activity during oogenesis
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEFINING NOVEL MECHANISMS REQUIRED FOR THE FORMATION AND MORPHOGENESIS OF A POLARIZED EPITHELIUM
oaire.awardTitleCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F37587%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BID%2F111796%2F2009/PT
oaire.citation.endPage291
oaire.citation.issue2
oaire.citation.startPage277
oaire.citation.titleDevelopmental Biology
oaire.citation.volume394
oaire.fundingStream3599-PPCDT
person.familyNameFerreira
person.familyNamePrudêncio
person.familyNameViegas Russo da Conceição Martinho
person.givenNameTania
person.givenNamePedro
person.givenNameRui Gonçalo
person.identifier.ciencia-idD61D-DA27-C815
person.identifier.ciencia-id7B1C-9F36-22C8
person.identifier.ciencia-idF31D-C783-3D89
person.identifier.orcid0000-0002-4175-9684
person.identifier.orcid0000-0003-1390-8491
person.identifier.orcid0000-0002-1641-3403
person.identifier.scopus-author-id57128656900
person.identifier.scopus-author-id54884299400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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