Publication
Influence of consecutive-day blood sampling on polymerase chain reaction-adjusted parasitological cure rates in an antimalarial-drug trial conducted in Tanzania
| dc.contributor.author | Martensson, Andreas | |
| dc.contributor.author | Ngasala, Billy | |
| dc.contributor.author | Ursing, Johan | |
| dc.contributor.author | Veiga, M. Isabel | |
| dc.contributor.author | Wiklund, Lisa | |
| dc.contributor.author | Membi, Christopher | |
| dc.contributor.author | Montgomery, Scott M. | |
| dc.contributor.author | Premji, Zul | |
| dc.contributor.author | Farnert, Anna | |
| dc.contributor.author | Bjorkman, Anders | |
| dc.date.accessioned | 2018-12-07T14:53:01Z | |
| dc.date.available | 2018-12-07T14:53:01Z | |
| dc.date.issued | 2007-02 | |
| dc.description.abstract | We assessed the influence that consecutive-day blood sampling, compared with single-day blood sampling, had on polymerase chain reaction (PCR)-adjusted parasitological cure after stepwise genotyping of merozoite surface proteins 2 (msp2) and 1 (msp1) in 106 children in Tanzania who had uncomplicated falciparum malaria treated with either sulfadoxine-pyrimethamine or artemether-lumefantrine; 78 of these children developed recurrent parasitemia during the 42-day follow-up period. Initial msp2 genotyping identified 27 and 33 recrudescences by use of single-and consecutive-day sampling, respectively; in subsequent msp1 genotyping, 17 and 21 of these episodes, respectively, were still classified as recrudescences; these results indicate a similar sensitivity of the standard single-day PCR protocol-that is, 82% (27/33) and 81% (17/21), in both genotyping steps. Interpretation of PCR-adjusted results will significantly depend on methodology. | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | |
| dc.identifier.doi | https://doi.org/10.1086/510910 | |
| dc.identifier.issn | 0022-1899 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/11317 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | University of Chicago Press | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Plasmodium falciparum malaria | |
| dc.subject | Dynamics | |
| dc.subject | Children | |
| dc.title | Influence of consecutive-day blood sampling on polymerase chain reaction-adjusted parasitological cure rates in an antimalarial-drug trial conducted in Tanzania | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 601 | |
| oaire.citation.issue | 4 | |
| oaire.citation.startPage | 597 | |
| oaire.citation.title | Journal of Infectious Diseases | |
| oaire.citation.volume | 195 | |
| rcaap.rights | openAccess | |
| rcaap.type | article |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Influence of consecutive-day blood sampling on polymerase.pdf
- Size:
- 316.15 KB
- Format:
- Adobe Portable Document Format