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Vaginal microbiota profile at the time of embryo transfer does not affect live birth rate in IVF cycles with donated oocytes

2019-06Journal article Restricted access
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dc.contributor.authorVergaro, Paula
dc.contributor.authorTiscornia, Gustavo
dc.contributor.authorBarragan, Montserrat
dc.contributor.authorGarcia, Desiree
dc.contributor.authorRodriguez, Amelia
dc.contributor.authorSantalo, Josep
dc.contributor.authorVassena, Rita
dc.date.accessioned2020-07-24T10:52:15Z
dc.date.available2020-07-24T10:52:15Z
dc.date.issued2019-06
dc.description.abstractResearch question: What is the relationship between the vaginal microbiota profile at the time of embryo transfer and live birth rates in women undergoing IVF/intracytoplasmic sperm injection (ICSI) with donated oocytes? Design: One hundred and fifty Caucasian women receiving donated oocytes were prospectively included in the study from March 2017 to January 2018. Samples of vaginal fluid were taken immediately before transfer of a fresh single blastocyst and genomic DNA (gDNA) was extracted. Bacterial load as well as the presence of four lactobacilli (L. crispatus, L. gasseri, L. jensenii and L. iners) and species associated with bacterial vaginosis (Gardnerella vaginalis, Atopobium vaginae, Mycoplasma hominis and Prevotella spp. - here collectively termed BVB) were determined by quantitative polymerase chain reaction. Vaginal microbiota profiles for each patient were characterized and correlated with reproductive results. Results: Although bacterial load was variable, a majority of samples were dominated by a single species (80.7%, 121/150). Most samples (76.7%, 115/150) were dominated by Lactobacillus spp., while 23.3% (35/150) were dominated by bacteria associated with bacterial vaginosis. The distribution of microbiota profiles among women who achieved a live birth and women who did not was similar (P = 0.43). Interestingly, we found a significantly higher proportion of samples dominated by L. crispatus- in women achieving live birth compared with those who did not (P = 0.021)
dc.description.abstractthis correlation was also statistically significant for biochemical pregnancy (P = 0.039) and clinical pregnancy (P = 0.015). Conclusions: Our data suggest that bacterial vaginosis-like vaginal microbiota at the time of embryo transfer does not directly affect the live birth rate.
dc.description.sponsorshipintramural funding of Clinica EUGIN
dc.description.sponsorshipMinistry of Economy and Knowledge of the Government of Catalonia [GENCAT 2015 DI 048]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.rbmo.2018.12.019
dc.identifier.issn1472-6483
dc.identifier.issn1472-6491
dc.identifier.urihttp://hdl.handle.net/10400.1/14337
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science
dc.subjectIn-vitro fertilization
dc.subjectPolymerase-chain-reaction
dc.subjectTotal bacterial load
dc.subject1St trimester
dc.subjectVaginosis
dc.subjectWomen
dc.subjectAssay
dc.subjectPcr
dc.subjectImplantation
dc.subjectMiscarriage
dc.titleVaginal microbiota profile at the time of embryo transfer does not affect live birth rate in IVF cycles with donated oocytes
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage891
oaire.citation.issue6
oaire.citation.startPage883
oaire.citation.titleReproductive BioMedicine Online
oaire.citation.volume38
person.familyNameTiscornia
person.givenNameGustavo
person.identifier.ciencia-id7818-59E3-E4EE
person.identifier.orcid0000-0002-1841-5330
person.identifier.scopus-author-id6507863404
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication8d2c4950-f020-4c0c-bbbd-52d19e1397a4
relation.isAuthorOfPublication.latestForDiscovery8d2c4950-f020-4c0c-bbbd-52d19e1397a4

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