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Identification of novel biomarkers in chronic immune thrombocytopenia (ITP) by microarray-based serum protein profiling

2016-02Journal article Open access
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dc.contributor.authorBal, Guerkan
dc.contributor.authorFutschik, Matthias E.
dc.contributor.authorHartl, Daniela
dc.contributor.authorRingel, Frauke
dc.contributor.authorKamhieh-Milz, Julian
dc.contributor.authorSterzer, Viktor
dc.contributor.authorHoheisel, Joerg D.
dc.contributor.authorAlhamdani, Mohamed S. S.
dc.contributor.authorSalama, Abdulgabar
dc.date.accessioned2017-04-07T15:57:11Z
dc.date.available2017-04-07T15:57:11Z
dc.date.issued2016-02
dc.description.abstractThe pathological mechanisms underlying the development of immune thrombocytopenia (ITP) are unclear and its diagnosis remains a process of exclusion. Currently, there are no known specific biomarkers for ITP to support differential diagnosis and treatment decisions. Profiling of serum proteins may be valuable for identifying such biomarkers. Sera from 46 patients with primary chronic ITP and 34 healthy blood donors were analysed using a microarray of 755 antibodies. We identified 161 differentially expressed proteins. In addition to oncoproteins and tumour-suppressor proteins, including apoptosis regulator BCL2, breast cancer type 1 susceptibility protein (BRCA1), Fanconi anaemia complementation group C (FANCC) and vascular endothelial growth factor A (VEGFA), we detected six anti-nuclear autoantibodies in a subset of ITP patients: anti-PCNA, anti-SmD, anti-Ro/SSA60, anti-Ro/SSA52, anti-La/SSB and anti-RNPC antibodies. This finding may provide a rational explanation for the association of ITP with malignancies and other autoimmune diseases. While RUNX1mRNA expression in the peripheral blood mononuclear cells (PBMC) of patients was significantly downregulated, an accumulation of RUNX1 protein was observed in the platelets of ITP patients. This may indicate dysregulation of RUNX1 expression in PBMC and megakaryocytes and may lead to an imbalanced immune response and impaired thrombopoiesis. In conclusion, we provide novel insights into the pathogenic mechanisms of ITP that warrant further exploration.
dc.identifier.doi10.1111/bjh.13861
dc.identifier.issn0007-1048
dc.identifier.otherAUT: MFU02241;
dc.identifier.urihttp://hdl.handle.net/10400.1/9639
dc.language.isoeng
dc.peerreviewedyes
dc.relationDeciphering the gene regulatory networks of cyanobacteria
dc.relationCentre for Biomedical Research
dc.relation.isbasedonWOS:000370000200011
dc.subjectChronic autoimmune thrombocytopenic purpura
dc.subjectCancer
dc.subjectB cell CLL/lymphoma 2
dc.subjectRunt-related transcription factor 3
dc.subjectThrombocytopenia
dc.titleIdentification of novel biomarkers in chronic immune thrombocytopenia (ITP) by microarray-based serum protein profiling
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDeciphering the gene regulatory networks of cyanobacteria
oaire.awardTitleCentre for Biomedical Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00881%2F2013%2FCP1198%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F04773%2F2013/PT
oaire.citation.endPage615
oaire.citation.issue4
oaire.citation.startPage602
oaire.citation.titleBritish Journal of Haematology
oaire.citation.volume172
oaire.fundingStreamInvestigador FCT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFutschik
person.givenNameMatthias
person.identifier.ciencia-idA71B-AD01-3501
person.identifier.orcid0000-0002-6245-8071
person.identifier.scopus-author-id14017989400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryd58f3269-c7e1-4c22-b094-5cfe6750821b
relation.isProjectOfPublication2994c09a-b440-45c8-abe0-8e0a883d29c6
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