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Mob1: defining cell polarity for proper cell division

2012-01Journal article Open Access
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dc.contributor.authorTavares, Alexandra
dc.contributor.authorGonçalves, João
dc.contributor.authorFlorindo, Claudia
dc.contributor.authorTavares, Alvaro A.
dc.contributor.authorSoares, Helena
dc.date.accessioned2018-12-07T14:53:09Z
dc.date.available2018-12-07T14:53:09Z
dc.date.issued2012-01
dc.description.abstractMob1 is a component of both the mitotic exit network and Hippo pathway, being required for cytokinesis, control of cell proliferation and apoptosis. Cell division accuracy is crucial in maintaining cell ploidy and genomic stability and relies on the correct establishment of the cell division axis, which is under the control of the cell's environment and its intrinsic polarity. The ciliate Tetrahymena thermophila possesses a permanent anterior posterior axis, left right asymmetry and divides symmetrically. These unique features of Tetrahymena prompted us to investigate the role of Tetrahymena Mob1. Unexpectedly, we found that Mob1 accumulated in basal bodies at the posterior pole of the cell, and is the first molecular polarity marker so far described in Tetrahymena. In addition, Mob1 depletion caused the abnormal establishment of the cell division plane, providing clear evidence that Mob1 is important for its definition. Furthermore, cytokinesis was arrested and ciliogenesis delayed in Tetrahymena cells depleted of Mob1. This is the first evidence for an involvement of Mob1 in cilia biology. In conclusion, we show that Mob1 is an important cell polarity marker that is crucial for correct division plane placement, for cytokinesis completion and for normal cilia growth rates.
dc.description.sponsorshipFundacao para a Ciencia e a Tecnologia (FCT) [PTDC/SAU-OBD/105234/2008]; Centro de Quimica e Bioquimica (CQB)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1242/jcs.096610
dc.identifier.issn0021-9533
dc.identifier.urihttp://hdl.handle.net/10400.1/11377
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany Of Biologists Ltd
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTetrahymena-Thermophila
dc.subjectMitotic exit
dc.subjectMacronuclear division
dc.subjectCycle progression
dc.subjectKinase complex
dc.subjectCytokinesis
dc.subjectFamily
dc.subjectMicrotubules
dc.subjectPosition
dc.subjectMitosis
dc.titleMob1: defining cell polarity for proper cell division
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F105234%2F2008/PT
oaire.citation.endPage527
oaire.citation.issue2
oaire.citation.startPage516
oaire.citation.titleJournal of Cell Science
oaire.citation.volume125
oaire.fundingStream3599-PPCDT
person.familyNameFlorindo
person.familyNameTavares
person.givenNameClaudia
person.givenNameAlvaro
person.identifier.ciencia-id7812-FEB4-622A
person.identifier.ciencia-idAD12-B7F1-969B
person.identifier.orcid0000-0003-4549-2179
person.identifier.orcid0000-0002-7137-0944
person.identifier.scopus-author-id26531090300
person.identifier.scopus-author-id19036533500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscovery4444e021-d38a-470f-b2d7-35fd2d7c1883
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