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Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles

2014-08Journal article Open Access
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dc.contributor.authorSilva, PMA; Patrícia M.A. Silva
dc.contributor.authorReis, Rita M.
dc.contributor.authorBolanos-Garcia, Victor M.
dc.contributor.authorFlorindo, Claudia
dc.contributor.authorTavares, Alvaro
dc.contributor.authorBousbaa, Hassan
dc.date.accessioned2018-12-07T14:52:45Z
dc.date.available2018-12-07T14:52:45Z
dc.date.issued2014-08
dc.description.abstractA predominant mechanism of spindle assembly checkpoint (SAC) silencing is dynein-mediated transport of certain kinetochore proteins along microtubules. There are still conflicting data as to which SAC proteins are dynein cargoes. Using two ATP reduction assays, we found that the core SAC proteins Mad1, Mad2, Bub1, BubR1, and Bub3 redistributed from attached kinetochores to spindle poles, in a dynein-dependent manner. This redistribution still occurred in metaphase-arrested cells, at a time when the SAC should be satisfied and silenced. Unexpectedly, we found that a pool of Hec1 and Mis12 also relocalizes to spindle poles, suggesting KMN components as additional dynein cargoes. The potential significance of these results for SAC silencing is discussed. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
dc.description.sponsorshipCESPU [02-GCQF-CICS-2011N]; national Portuguese funding through FCT - Fundacao para a Ciencia e a Tecnologia FCT [POCTI/BIA/PRO/60337/2004, PTDC/SAU-OBD/105234/2008]; FCT [PEst-OE/ EQB/LA0023/2013, SFRH/BD/90744/2012]; [EXPL/BEX-BCM/1104/2013]
dc.identifier.doihttps://doi.org/10.1016/j.febslet.2014.07.011
dc.identifier.issn0014-5793
dc.identifier.urihttp://hdl.handle.net/10400.1/11191
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectMitotic checkpoint
dc.subjectMicrotubule attachment
dc.subjectChromosome alignment
dc.subjectBub3 stability
dc.subjectLiving cells
dc.subjectComplex
dc.subjectApc/c
dc.subjectInhibition
dc.subjectCdc20
dc.subjectHec1
dc.titleDynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-PRO%2F60337%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F105234%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBEX-BCM%2F1104%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F90744%2F2012/PT
oaire.citation.endPage3273
oaire.citation.issue17
oaire.citation.startPage3265
oaire.citation.titleFebs Letters
oaire.citation.volume588
oaire.fundingStreamPOCI
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNameTavares
person.givenNamePatrícia
person.givenNameAlvaro
person.identifier1445798
person.identifier.ciencia-id4B19-7E26-A047
person.identifier.ciencia-idAD12-B7F1-969B
person.identifier.orcid0000-0002-0694-7321
person.identifier.orcid0000-0002-7137-0944
person.identifier.scopus-author-id55855611900
person.identifier.scopus-author-id19036533500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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