Repository logo
 
Publication

CITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells

2016-12Journal article Open access
Simple item page
dc.contributor.authorPacheco-Leyva, Ivette
dc.contributor.authorMatias, Ana Catarina
dc.contributor.authorOliveira, Daniel V.
dc.contributor.authorSantos, João
dc.contributor.authorNascimento, Rita
dc.contributor.authorGuerreiro, Eduarda
dc.contributor.authorMichell, Anna C.
dc.contributor.authorvan De Vrugt, Annebel M.
dc.contributor.authorMachado-Oliveira, Gisela
dc.contributor.authorFerreira, Guilherme
dc.contributor.authorDomian, Ibrahim
dc.contributor.authorBragança, José
dc.date.accessioned2017-04-07T15:55:34Z
dc.date.available2017-04-07T15:55:34Z
dc.date.issued2016-12
dc.description.abstractThe transcriptional regulator CITED2 is essential for heart development. Here, we investigated the role of CITED2 in the specification of cardiac cell fate from mouse embryonic stem cells (ESC). The overexpression of CITED2 in undifferentiated ESC was sufficient to promote cardiac cell emergence upon differentiation. Conversely, the depletion of Cited2 at the onset of differentiation resulted in a decline of ESC ability to generate cardiac cells. Moreover, loss of Cited2 expression impairs the expression of early mesoderm markers and cardiogenic transcription factors (Isl1, Gata4, Tbx5). The cardiogenic defects in Cited2-depleted cells were rescued by treatment with recombinant CITED2 protein. We showed that Cited2 expression is enriched in cardiac progenitors either derived from ESC or mouse embryonic hearts. Finally, we demonstrated that CITED2 and ISL1 proteins interact physically and cooperate to promote ESC differentiation toward cardiomyocytes. Collectively, our results show that Cited2 plays a pivotal role in cardiac commitment of ESC.
dc.identifier.doihttps://doi.org/10.1016/j.stemcr.2016.10.002
dc.identifier.issn2213-6711
dc.identifier.otherAUT: JEB02317;
dc.identifier.urihttp://hdl.handle.net/10400.1/9164
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationNOVEL HUMAN NEURAL IN VITRO MODEL FOR PRE-CLINICAL RESEARCH AND DRUG DEVELOPMENT
dc.relation.isbasedonWOS:000393188500003
dc.subjectTranscriptional regulation
dc.subjectCardiac progenitor
dc.subjectCited2
dc.subjectIsl1
dc.subjectEmbryonic stem cell
dc.subjectDifferentiation
dc.titleCITED2 cooperates with ISL1 and promotes cardiac differentiation of mouse embryonic stem cells
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNOVEL HUMAN NEURAL IN VITRO MODEL FOR PRE-CLINICAL RESEARCH AND DRUG DEVELOPMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-ENB%2F111702%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F74807%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F62054%2F2009/PT
oaire.citation.endPage1049
oaire.citation.issue6
oaire.citation.startPage1037
oaire.citation.titleStem Cell Reports
oaire.citation.volume7
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameMatias
person.familyNameSantos
person.familyNameBragança
person.givenNameAna Catarina
person.givenNameJoão
person.givenNameJosé
person.identifier2373709
person.identifier.ciencia-id701D-019D-0779
person.identifier.ciencia-idB713-B787-0A9E
person.identifier.ciencia-idAC1D-FA9D-F66F
person.identifier.orcid0000-0002-6385-9117
person.identifier.orcid0000-0002-1218-368X
person.identifier.orcid0000-0001-9566-400X
person.identifier.ridA-9430-2014
person.identifier.scopus-author-id55232110200
person.identifier.scopus-author-id6602220001
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication2b71c7a8-f595-4ca3-ba39-5553c33deb3c
relation.isAuthorOfPublication8d796747-aa87-473b-8dec-35d27b95c7b9
relation.isAuthorOfPublication27334e02-e955-4939-b9b5-bdee5b5f9328
relation.isAuthorOfPublication.latestForDiscovery27334e02-e955-4939-b9b5-bdee5b5f9328
relation.isProjectOfPublicatione13142f2-37b8-4b5a-b2cd-352e62003184
relation.isProjectOfPublication89fcacba-95e1-424f-aee5-1a0bdc3d7303
relation.isProjectOfPublication9c2d9344-6ca3-4194-8776-6c6bcb0a97ca
relation.isProjectOfPublication60fae661-67b2-4270-8938-5a1720a32fb3
relation.isProjectOfPublication21010513-d635-427a-81ec-3916519d9adc
relation.isProjectOfPublication.latestForDiscovery89fcacba-95e1-424f-aee5-1a0bdc3d7303

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
9164.pdf
Size:
2.55 MB
Format:
Adobe Portable Document Format
Download

Collections

FCB2-Artigos (em revistas ou actas indexadas)