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Xenopus Pkdcc1 and Pkdcc2 Are Two New Tyrosine Kinases Involved in the Regulation of JNK Dependent Wnt/PCP Signaling Pathway

2015-08Journal article Open access
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dc.contributor.authorVitorino, Marta
dc.contributor.authorSilva, Ana Cristina
dc.contributor.authorInacio, Jose Manuel
dc.contributor.authorRamalho, Jose Silva
dc.contributor.authorGur, Michal
dc.contributor.authorFainsod, Abraham
dc.contributor.authorSteinbeisser, Herbert
dc.contributor.authorBelo, José A.
dc.date.accessioned2018-12-07T14:52:44Z
dc.date.available2018-12-07T14:52:44Z
dc.date.issued2015-08
dc.description.abstractProtein Kinase Domain Containing, Cytoplasmic (PKDCC) is a protein kinase which has been implicated in longitudinal bone growth through regulation of chondrocytes formation. Nevertheless, the mechanism by which this occurs remains unknown. Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are both involved on blastopore and neural tube closure during gastrula and neurula stages, respectively. In vertebrates, tissue polarity and cell movement observed during gastrulation and neural tube closure are controlled by Wnt/Planar Cell Polarity (PCP) molecular pathway. Our results showed that Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane. But surprisingly, they revealed different roles in the induction of a luciferase reporter under the control of Atf2 promoter. While Pkdcc1 induces Atf2 expression, Pkdcc2 does not, and furthermore inhibits its normal induction by Wnt11 and Wnt5a. Altogether our data show, for the first time, that members of the PKDCC family are involved in the regulation of JNK dependent Wnt/PCP signaling pathway.
dc.description.sponsorshipFundacao Ciencia e Tecnologia - IP; IBB/CBME, LA [PTDC/BIA-BCM/69912/2006, Pest-OE/EQB/LA0023/2013]; FCT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pone.0135504
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11185
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPlanar cell polarity
dc.subjectNeural-tube closure
dc.subjectAnterior primitive endoderm
dc.subjectConvergent extension
dc.subjectVertebrate gastrulation
dc.subjectApical constriction
dc.subjectSecreted factor
dc.subjectPcp Pathway
dc.subjectWnt
dc.subjectProtein
dc.titleXenopus Pkdcc1 and Pkdcc2 Are Two New Tyrosine Kinases Involved in the Regulation of JNK Dependent Wnt/PCP Signaling Pathway
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F69912%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/Incentivo%2FEQB%2FLA0023%2F2013/PT
oaire.citation.issue8
oaire.citation.startPagee0135504
oaire.citation.titlePlos One
oaire.citation.volume10
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameVitorino
person.familyNameInacio
person.familyNameBelo
person.givenNameMarta
person.givenNameJose M
person.givenNameJosé A.
person.identifier.ciencia-id4615-FF5B-E951
person.identifier.ciencia-idB810-3281-8D8C
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0001-8563-8975
person.identifier.orcid0000-0002-9790-8335
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridU-9947-2018
person.identifier.ridO-1455-2018
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6604046494
person.identifier.scopus-author-id6602141392
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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