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Aliskiren decreases oxidative stress and angiogenic markers in retinal pigment epithelium cells

2017-02Journal article Restricted access
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dc.contributor.authorS, Simão
dc.contributor.authorSantos, Daniela F.
dc.contributor.authorSilva, Gabriela A.
dc.date.accessioned2019-11-20T15:07:49Z
dc.date.available2019-11-20T15:07:49Z
dc.date.issued2017-02
dc.description.abstractThere is growing evidence on the role of ocular renin-angiotensin system (RAS) in the development of diabetic retinopathy (DR), particularly due to the trigger of oxidative stress and angiogenesis. Despite this there is no effective RAS-based therapy in DR capable of preventing retinal damage induced by RAS activation. We recently described that retinal pigment epithelium (RPE) cells express the main components of the RAS. We here propose to investigate the role of glucose upon the retinal RAS and whether aliskiren, a direct renin inhibitor, protects RPE cells from angiogenesis and oxidative stress. RPE cells were chosen as target since one of the first events in DR is the dysfunction of the RPE retinal layer, which as a key function in maintaining the integrity of the retina. We found that the RAS present in the RPE cells was deregulated by hyperglycemic glucose concentrations. Exposure of RPE cells to angiotensin II increased the levels of the main pro-angiogenic factor, vascular endothelial growth factor (VEGF) in a concentration-dependent manner. Additionally, angiotensin II also stimulated the production of reactive oxygen species in RPE cells. Treatment of RPE cells with aliskiren decreased the levels of oxidative stress and promoted the expression of anti-angiogenic factors such as the pigment epithelium-derived factor and the VEGF(165)b isoform. Our findings demonstrate that the RAS is deregulated in hyperglycemic conditions and that aliskiren successfully protected RPE cells from RAS over activation. These anti-angiogenic and antioxidant properties described for aliskiren over RPE cells suggest that this drug has potential to be used in the treatment of diabetic retinopathy.
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT)
dc.description.sponsorshipResearch Center Grant [UID/BIM/04773/2013, iNOVA4-Health-UID/Multi/04462/2013]
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia/Ministerio da Educacao e Ciencia, through national funds
dc.description.sponsorshipFEDER under the PT2020 Partnership Agreement
dc.description.sponsorship[SFRH/BPD/78404/2011]
dc.description.sponsorship[PD/BD/114251/2016]
dc.description.sponsorship[EXPL-BIM-MEC-1433-2013]
dc.description.sponsorship[PIRG05-GA-2009-249314-EyeSee]
dc.identifier.doi10.1007/s10456-016-9526-5
dc.identifier.issn0969-6970
dc.identifier.issn1573-7209
dc.identifier.urihttp://hdl.handle.net/10400.1/13233
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.subjectRenin-Angiotensin System
dc.subjectDiabetic-Retinopathy
dc.subjectSplice Variant
dc.subjectVegf
dc.subjectExpression
dc.subjectBlockade
dc.subjectNeovascularization
dc.subjectVegf(165)B
dc.subjectReceptor
dc.subjectMice
dc.titleAliskiren decreases oxidative stress and angiogenic markers in retinal pigment epithelium cells
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78404%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04462%2F2013/PT
oaire.citation.endPage181
oaire.citation.issue1
oaire.citation.startPage175
oaire.citation.titleAngiogenesis
oaire.citation.volume20
oaire.fundingStreamSFRH
oaire.fundingStream5876
person.familyNameSimao
person.givenNameSonia
person.identifier.orcid0000-0002-0245-0633
person.identifier.scopus-author-id24067982400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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