Publication
Coding and non-coding RNA expression in NSC34 cells following TDP-43 depletion and mutant TDP-43 M337V expression
| dc.contributor.author | Gbadamosi, Ismail | |
| dc.contributor.author | Binias, Sandra | |
| dc.contributor.author | Gielniewski, Bartłomiej | |
| dc.contributor.author | Magno, Ramiro | |
| dc.contributor.author | dos Santos Duarte, Guilhermina Isabel | |
| dc.contributor.author | Jawaid, Ali | |
| dc.date.accessioned | 2025-09-13T11:54:17Z | |
| dc.date.available | 2025-09-13T11:54:17Z | |
| dc.date.issued | 2025-07-01 | |
| dc.description.abstract | Several neurodegenerative disorders (NDDs), notably amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) are characterized by pathological cytoplasmic aggregation of TAR DNA-binding protein 43 (TDP-43) in neurons and glia. Primarily localized in the nucleus under physiological conditions, TDP-43 is a critical regulator of RNA processing and metabolism. Therefore, RNA changes induced by TDP-43 depletion or mutation could play an important role in the pathogenesis of ALS and other TDP-43 related NDDs.To investigate these effects in NSC34 motor neuron-like cells, a commonly used cellular model of ALS, we used RNA interference to knock down TDP-43 and overexpressed the ALS-associated TDP-43 M337V mutation. RNA from both these experiments was enriched for small and large transcripts and subsequently analyzed via next-generation sequencing. The resulting transcriptomics datasets offer a valuable resource for studying the impact of TDP-43 depletion and mutant over-expression in motor neurons. These data enable comprehensive differential expression analyses and functional enrichment studies, identifying cellular pathways affected by TDP43 depletion or mutation. Additionally, the inclusion of non-coding RNAs facilitates the construction of gene regulatory networks, providing insights into the interplay between coding and non-coding RNAs in gene expression regulation under TDP-43 loss-of-function or pathogenic mutation conditions. | eng |
| dc.description.sponsorship | UMO-2022/04/Y/NZ5/00122 | |
| dc.identifier.doi | 10.1038/s41597-025-05409-7 | |
| dc.identifier.issn | 2052-4463 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/27690 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Springer | |
| dc.relation.ispartof | Scientific Data | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.title | Coding and non-coding RNA expression in NSC34 cells following TDP-43 depletion and mutant TDP-43 M337V expression | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 1 | |
| oaire.citation.title | Scientific Data | |
| oaire.citation.volume | 12 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | dos Santos Duarte | |
| person.givenName | Guilhermina Isabel | |
| person.identifier.ciencia-id | ED15-7F67-7B64 | |
| person.identifier.orcid | 0000-0003-0060-2936 | |
| relation.isAuthorOfPublication | 6e1e1dd4-e2b4-4dfa-b7d7-f7990e9bd179 | |
| relation.isAuthorOfPublication.latestForDiscovery | 6e1e1dd4-e2b4-4dfa-b7d7-f7990e9bd179 |