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Thermal inactivation of Alyciclobacillus acidoterrestris spores under different temperature, soluble solids and pH conditions for the design of fruit processes

dc.contributor.authorSilva, F. M.
dc.contributor.authorGibbs, P.
dc.contributor.authorSilva, C. L. M.
dc.contributor.authorVieira, M. M. C.
dc.date.accessioned2013-03-05T15:55:07Z
dc.date.available2013-03-05T15:55:07Z
dc.date.issued1999-10
dc.description.abstractAlicyclobacillus acidoterrestris, a thermoacidophilic, non-pathogenic and spore-forming bacterium has been detected in several spoiled commercial pasteurised fruit juices. A. acidoterrestris spores, besides being resistant to the pasteurisation treatment conditions normally applied to acidic fruit products, can germinate and grow causing spoilage. Therefore, this microorganism was suggested as the target to be used in the design of adequate pasteurisation processes. The objectives of this work were to investigate the influence of temperature (T: 85–97°C), total soluble solids (SS: 5–60°Brix or % by weight) and pH (2.5–6.0) on D-values (decimal reduction time) of Alicyclobacillus acidoterrestris (type strain, NCIMB 13137) spores, and to fit a model using response surface methodology. A central composite face-centred experimental design was used, and the response, D-value determined in malt extract broth, ranged between 0.498±0.045 and 94.9±6.7 min. Within the factor ranges studied, temperature was the parameter that most affected the D-value. Following this was the SS and, lastly, the pH value. A linear decrease in D-value was observed with decreasing SS and pH, and a non-linear decrease in D-value was noticed with increasing temperature. A second order polynomial was successfully fitted to the data (R2=0.98). In general, D-values measured in real fruit systems, such as orange, apple and grape juices, blackcurrant concentrates, cupuaçu (exotic fruit) extract and orange juice drink, were higher than those predicted by the malt extract broth model. This result emphasises the importance of experimental validation of any model-derived process.por
dc.description.sponsorshipContract STD no. ERB-TS3-CT94-0300
dc.identifier.citationSilva, F.M., Gibbs, P., Vieira, M.C. and Silva, C.L.M. (1999). Thermal inactivation of Alyciclobacillus acidoterrestris spores under different temperature, soluble solids and pH conditions for the design of fruit processes. International Journal of Food Microbiology. 51, 95-103por
dc.identifier.doihttp://dx.doi.org/10.1016/S0168-1605(99)00103-8
dc.identifier.otherAUT: MVI00201;
dc.identifier.urihttp://hdl.handle.net/10400.1/2536
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0168160599001038por
dc.subjectResponse surface methodologypor
dc.subjectAlyciclobacillus acidoterrestris sporespor
dc.subjectThermal inactivationpor
dc.subjectHeat resistancepor
dc.subjectFruit productspor
dc.subjectKineticspor
dc.titleThermal inactivation of Alyciclobacillus acidoterrestris spores under different temperature, soluble solids and pH conditions for the design of fruit processespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage103por
oaire.citation.issue2-3por
oaire.citation.startPage95por
oaire.citation.titleInternational Journal of Food Microbiologypor
oaire.citation.volume51por
person.familyNameVieira
person.givenNameMargarida
person.identifier.ciencia-id3314-4EFC-1E4A
person.identifier.orcid0000-0003-2774-706X
person.identifier.ridE-1805-2012
person.identifier.scopus-author-id7202140775
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication338c2b9c-fbe6-4fc1-af2d-8152a6560119
relation.isAuthorOfPublication.latestForDiscovery338c2b9c-fbe6-4fc1-af2d-8152a6560119

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