Repository logo
 
Publication

Production of MLM-Type structured lipids catalyzed by immobilized heterologous rhizopus oryzae lipase

2011Journal article Restricted access
Simple item page
dc.contributor.authorNunes, Patrícia A.
dc.contributor.authorCabral, Paula Pires
dc.contributor.authorGuillen, M.
dc.contributor.authorValero, F.
dc.contributor.authorLuna, D.
dc.contributor.authorDias, S. Ferreira
dc.date.accessioned2016-02-03T16:15:08Z
dc.date.available2016-02-03T16:15:08Z
dc.date.issued2011
dc.description.abstractThis work aims to produce triacylglycerols (TAG) containing a medium-chain fatty acid (M) at positions sn-1,3 and a long-chain fatty acid (L) at sn-2 position, i.e. TAG of MLM type, by acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, catalyzed by 1,3-selective Rhizopus oryzae heterologous lipase (rROL) immobilized in Eupergit (R) C and modified sepiolite. This lipase was produced by the methylotrophic yeast Pichia pastoris. Reactions were performed at 25 and 40 degrees C, for 24 h, either in solvent-free or in n-hexane media, at a molar ratio 1:2 (olive oil:free fatty acid). Higher incorporations of C8:0 (21.6 mol%) and C10:0 (34.8 mol%) into the TAG were attained in solvent-free media, at 40 degrees C, when rROL immobilized in Eupergit (R) C was used. In organic media, at 40 degrees C, only 15.9 and 14.1 mol%, incorporation of C8:0 or C10:0 were, respectively observed. Lower incorporations were attained for both acids (3.4-7.0 mol%) when native ROL (nROL) in both supports and rROL in modified sepiolite were used. rROL in Eupergit (R) C maintained its activity during the first four or three 23-h batches, respectively when C8:0 (half-life time, t(1/2) = 159 h) or C10:0 (t(1/2) = 136 h) were used, decreasing thereafter following a time delay model.
dc.identifier.doihttps://dx.doi.org/10.1007/s11746-010-1702-y
dc.identifier.issn0003-021X
dc.identifier.otherAUT: PCA00486;
dc.identifier.urihttp://hdl.handle.net/10400.1/7588
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relation.isbasedonP-002-SMN
dc.titleProduction of MLM-Type structured lipids catalyzed by immobilized heterologous rhizopus oryzae lipase
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage480
oaire.citation.startPage473
oaire.citation.titleJournal of the American Oil Chemists' Society
oaire.citation.volume88
person.familyNameNunes Cabrita
person.givenNamePatrícia
person.identifier.orcid0000-0002-7148-8950
person.identifier.scopus-author-id7006823084
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b0b5899-4a35-4405-acbe-a8f548457da1
relation.isAuthorOfPublication.latestForDiscovery4b0b5899-4a35-4405-acbe-a8f548457da1

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Production of MLM-Type Structured Lipids Catalyzed by Immobilized Heterologous Rhizopus oryzae Lipase.pdf
Size:
277.97 KB
Format:
Adobe Portable Document Format
Download

Collections