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Listeria monocytogenes Has Both Cytochrome bd-Type and Cytochrome aa(3)-Type Terminal Oxidases, Which Allow Growth at Different Oxygen Levels, and Both Are Important in Infection

2017-11Journal article Open access
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dc.contributor.authorCorbett, David
dc.contributor.authorGoldrick, Marie
dc.contributor.authorFernandes, Vitor E.
dc.contributor.authorDavidge, Kelly
dc.contributor.authorPoole, Robert K.
dc.contributor.authorAndrew, Peter W.
dc.contributor.authorCavet, Jennifer
dc.contributor.authorRoberts, Ian S.
dc.date.accessioned2018-12-07T14:57:55Z
dc.date.available2018-12-07T14:57:55Z
dc.date.issued2017-11
dc.description.abstractListeria monocytogenes is a foodborne pathogen responsible for a number of life-threatening infections of humans. During an infection, it invades epithelial cells before spreading from the intestine to the cells of the liver and spleen. This requires an ability to adapt to varying oxygen levels. Here, we demonstrate that L. monocytogenes has two terminal oxidases, a cytochrome bd-type (CydAB) and a cytochrome aa(3)-type menaquinol (QoxAB) oxidase, and that both are used for respiration under different oxygen tensions. Furthermore, we show that possession of both terminal oxidases is important in infection. In air, the CydAB bd-type oxidase is essential for aerobic respiration and intracellular replication, and cydAB mutants are highly attenuated in mice. In contrast, the QoxAB aa(3)-type oxidase is required neither for aerobic respiration in air nor for intracellular growth. However, the qoxAB mutants are attenuated in mice, with a delay in the onset of disease signs and with increased survival time, indicating a role for the QoxAB aa(3)-type oxidase in the initial stages of infection. Growth of bacteria under defined oxygen conditions revealed that at 1% (vol/vol), both oxidases are functional, and the presence of either is sufficient for aerobic respiration and intracellular replication. However, at 0.2% (vol/vol), both oxidases are necessary for maximum growth. These findings are consistent with the ability of L. monocytogenes to switch between terminal oxidases under different oxygen conditions, providing exquisite adaptation to different conditions encountered within the infected host.
dc.description.sponsorshipMRC [G0601205]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1128/IAI.00354-17
dc.identifier.issn0019-9567
dc.identifier.issn1098-5522
dc.identifier.urihttp://hdl.handle.net/10400.1/11766
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Society for Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectActin-based motility
dc.subjectEscherichia-Coli
dc.subjectAzotobacter-Vinelandii
dc.subjectQuinol oxidase
dc.subjectIn-Vitro
dc.subjectVirulence
dc.subjectExpression
dc.subjectSurvival
dc.subjectComplex
dc.subjectGenes
dc.titleListeria monocytogenes Has Both Cytochrome bd-Type and Cytochrome aa(3)-Type Terminal Oxidases, Which Allow Growth at Different Oxygen Levels, and Both Are Important in Infection
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11
oaire.citation.startPagee00354-17
oaire.citation.titleInfection and Immunity
oaire.citation.volume85
person.familyNameE. Fernandes
person.givenNameVitor
person.identifier.ciencia-id3811-AC60-F24D
person.identifier.orcid0000-0001-5179-2122
person.identifier.scopus-author-id36155199000
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationebeb7825-b898-4b10-85b9-28915399dde1
relation.isAuthorOfPublication.latestForDiscoveryebeb7825-b898-4b10-85b9-28915399dde1

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