Repository logo
 
Publication

The renin-angiotensin system in healthy human platelets: expressed but inactive

2025-08-18Journal article Open access
Simple item page
dc.contributor.authorPanosetti, François
dc.contributor.authorCuenot, François M.
dc.contributor.authorAuguste, Damian S. Saint
dc.contributor.authorMartins Cavaco, Ana Cláudia
dc.contributor.authorNunes, Allancer D. C.
dc.contributor.authorLu, Philip H. J.
dc.contributor.authorMagrini, Céline
dc.contributor.authorMolot, Max
dc.contributor.authorSanglard, Gabriel
dc.contributor.authorGünçü, Rodi
dc.contributor.authorZouaghi, Yassine
dc.contributor.authorBéguelin, Charles
dc.contributor.authorLima, Augusto Martins
dc.contributor.authorStergiopulos, Nikolaos
dc.date.accessioned2025-09-25T13:26:00Z
dc.date.available2025-09-25T13:26:00Z
dc.date.issued2025-08-18
dc.description.abstractPlatelets play a crucial role in arterial thrombus formation, offering potential for new antiplatelet therapies with reduced bleeding risk. Here, we investigated the role of the renin-angiotensin system (RAS) in human platelets and explored its potential link to COVID-19 coagulopathy. Experiments were performed ex vivo on healthy human platelets. The expression of RAS receptors (Mas, MrgD, ACE, ACE2, AT1 and AT2) was evaluated using western blot and immunofluorescence. Platelets were incubated in vitro with either Captopril or different RAS peptides including Alamandine, Angiotensin-I, Angiotensin-II, Angiotensin-(1-7), and Angiotensin-(1-9). Platelet adhesion was measured by spectrophotometry using BCECF fluorescence. Platelet activation and aggregation were analyzed using aggregometry after stimulation with extracellular matrix proteins. ACE and ACE2 activity were assessed using Fluorescent Peptides (FPS). We demonstrated that healthy human platelets express all the tested RAS receptors. However, RAS peptides did not modulate platelet adhesion or aggregation despite a wide range of concentrations tested. ACE activity was detected in platelet lysates, but it was not inhibited by Captopril, while ACE2 activity was undetectable. Our findings suggest that while RAS receptors are expressed in platelets, RAS peptides do not impact platelet function, at least in our experimental setting. COVID-19 coagulopathy may occur independently of the RAS.eng
dc.identifier.doi10.1080/09537104.2025.2546982
dc.identifier.eissn1369-1635
dc.identifier.issn0953-7104
dc.identifier.urihttp://hdl.handle.net/10400.1/27740
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInforma
dc.relation.ispartofPlatelets
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectACE2
dc.subjectAdhesion
dc.subjectAggregation
dc.subjectCovid-19
dc.subjectHuman platelet
dc.subjectRenin-angiotensin system
dc.titleThe renin-angiotensin system in healthy human platelets: expressed but inactiveeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.titlePlatelets
oaire.citation.volume36
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMartins Cavaco
person.givenNameAna Cláudia
person.identifier.orcid0000-0002-0642-1979
relation.isAuthorOfPublication84ee62df-e553-44f2-9336-ab736f501793
relation.isAuthorOfPublication.latestForDiscovery84ee62df-e553-44f2-9336-ab736f501793

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
The renin-angiotensin system in healthy human platelets expressed but inactive.pdf
Size:
9.49 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCB2-Artigos (em revistas ou actas indexadas)